Overview
- External ontology
- komodo.medium:1121 (source link unavailable) — MMJS MEDIUM (modified)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 1121 | DSMZ Medium: 1121 (mediadive.medium:1121) | Aerobic: No
Composition
flowchart LR
CultureMech_003824["mmjs_medium_modified"]
ing0_NaCl["NaCl"]
CultureMech_003824 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_003824 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_003824 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003824 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_003824 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003824 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003824 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_003824 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Fe_III__citrate["Fe(III) citrate"]
CultureMech_003824 --> ing8_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing8_Fe_III__citrate -.-> CHEBI_144421
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003824 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_003824 --> ing10_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing11_Sulfur["Sulfur"]
CultureMech_003824 --> ing11_Sulfur
CHEBI_26833["CHEBI:26833"]
ing11_Sulfur -.-> CHEBI_26833
ing12_NaHCO3["NaHCO3"]
CultureMech_003824 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003824 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003824 --> ing14_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003824 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003824 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003824 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003824 --> ing18_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing19_H3BO3["H3BO3"]
CultureMech_003824 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003824 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_003824 --> ing21_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing21_SrCl2_x_6_H2O -.-> CHEBI_36385
ing22_NaBr["NaBr"]
CultureMech_003824 --> ing22_NaBr
CHEBI_63004["CHEBI:63004"]
ing22_NaBr -.-> CHEBI_63004
ing23_KI["KI"]
CultureMech_003824 --> ing23_KI
CHEBI_8346["CHEBI:8346"]
ing23_KI -.-> CHEBI_8346
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003824 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003824 --> ing25_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing26_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
CultureMech_003824 --> ing26_Fe2_SO4_3_x_n_H2O
CHEBI_53438["CHEBI:53438"]
ing26_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
ing27_H2WO4["H2WO4"]
CultureMech_003824 --> ing27_H2WO4
CHEBI_36272["CHEBI:36272"]
ing27_H2WO4 -.-> CHEBI_36272
ing28_Biotin["Biotin"]
CultureMech_003824 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Folic_acid["Folic acid"]
CultureMech_003824 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_003824 --> more["...9 more"]
Ingredients (39)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.09 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.07 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.01 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
3 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 12 |
Sulfur
S 32.067 g/mol
|
3 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.5 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.001 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.01 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 23 |
NaBr
Br.Na 102.894 g/mol
|
0.01 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 24 |
KI
I.K 166.002 g/mol
|
0.01 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.1 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 27 |
Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
|
1 G_PER_L |
CHEBI:53438
iron(3+) sulfate
|
|
| 28 |
H2WO4
H2O4W 249.852 g/mol
|
0.1 G_PER_L |
CHEBI:36272
tungstic acid
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 35 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 36 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 37 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 38 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 39 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_1121_MMJS_MEDIUM_modified (CultureMech:003824)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1121 |
| 2026-02-03T11:19:17.330374 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1121 |
| 2026-03-13T03:09:37.725855Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:22.535101Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003824 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:36.938254+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJS MEDIUM (modified)" to "mmjs_medium_modified" for consistent matching |
| 2026-05-16T09:03:00.196635+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:19.161426+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 37 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.547705+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:40.264196+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3da35bcba1e3041810c7b8c7d94f375d8bab85f826dbf0f66c8074b09a3e9882 |