Overview
- External ontology
- komodo.medium:1135 (source link unavailable) — HD-MEDIUM, 1:10 diluted, modified
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 1135 | DSMZ Medium: 1135 (mediadive.medium:1135) | Aerobic: No
Composition
flowchart LR
CultureMech_003844["hd_medium_1_10_diluted_modified"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_003844 --> ing0_Casein_peptone
ing1_Glucose["Glucose"]
CultureMech_003844 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Yeast_extract["Yeast extract"]
CultureMech_003844 --> ing2_Yeast_extract
ing3_MES["MES"]
CultureMech_003844 --> ing3_MES
CHEBI_39010["CHEBI:39010"]
ing3_MES -.-> CHEBI_39010
ing4_Double_distilled_water["Double distilled water"]
CultureMech_003844 --> ing4_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Double_distilled_water -.-> CHEBI_15377
ing5_Agar["Agar"]
CultureMech_003844 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_NaOH["NaOH"]
CultureMech_003844 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 0.5 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 | Yeast extract | 0.25 G_PER_L | ||
| 4 | MES | 1.95 G_PER_L |
CHEBI:39010
MES
|
|
| 5 |
Double distilled water
H2O 18.015 g/mol
|
2000.0 G_PER_L |
CHEBI:15377
water
|
[Merged 3 duplicates: 1000.0, 500.0, 500.0] |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_1135_HD-MEDIUM_1_10_diluted_modified (CultureMech:003844)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1135 |
| 2026-02-03T11:19:19.217093 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1135 |
| 2026-03-13T03:09:43.125148Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s); Added pH buffer: NaOH |
| 2026-03-13T06:19:22.670095Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003844 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:37.092083+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HD-MEDIUM, 1:10 diluted, modified" to "hd_medium_1_10_diluted_modified" for consistent matching |
| 2026-05-16T09:03:00.265076+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:19.253968+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:40.432688+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b70245a9d836486db849716dc06673c574370a17b834cded4e65254e767e97ef |