Overview
- External ontology
- komodo.medium:1205 (source link unavailable) — 1/2 TRYPTIC SOY BROTH (ANAEROBE)
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- pH buffer: HCl | Source: KOMODO ModelSEED | ID: 1205 | DSMZ Medium: 1205 (mediadive.medium:1205) | Aerobic: No Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_003953["1_2_tryptic_soy_broth_anaerobe"]
ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_003953 --> ing0_Pancreatic_digest_of_casein
ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_003953 --> ing1_Peptic_digest_of_soybean_meal
ing2_Glucose["Glucose"]
CultureMech_003953 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_003953 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_003953 --> ing4_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Dipotassium_phosphate -.-> CHEBI_131527
ing5_Agar["Agar"]
CultureMech_003953 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Yeast_extract["Yeast extract"]
CultureMech_003953 --> ing6_Yeast_extract
ing7_NaCl["NaCl"]
CultureMech_003953 --> ing7_NaCl
ing7_NaCl -.-> CHEBI_26710
ing8_D_Glucose["D-Glucose"]
CultureMech_003953 --> ing8_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing8_D_Glucose -.-> CHEBI_17634
ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_003953 --> ing9_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing9_DL_Dithiothreitol -.-> CHEBI_18320
ing10_HCl["HCl"]
CultureMech_003953 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 2 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 3 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 5 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 | Yeast extract | 1 G_PER_L | ||
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 |
D-Glucose
C6H12O6 180.156 g/mol
|
3.6 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 10 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
0.15 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 1_2_tryptic_soy_broth_anaerobe (CultureMech:003953)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1205 |
| 2026-02-03T11:19:07.190209 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1205 |
| 2026-03-13T03:09:11.369853Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: HCl |
| 2026-03-13T06:19:23.598608Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003953 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:38.185357+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/2 TRYPTIC SOY BROTH (ANAEROBE)" to "1_2_tryptic_soy_broth_anaerobe" for consistent matching |
| 2026-05-16T09:02:58.110283+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:15.561139+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:33.767381+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 47b64747b32f3d6885853f0a440e964b2ae7481db0b7b74199be87e82517bb62 |