Overview
- External ontology
- komodo.medium:1285 (source link unavailable) — GRANULICELLA PALUDICOLA medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 4.0, 'max': 5.0}
- Notes
- pH buffer: alginic acid | Source: KOMODO ModelSEED | ID: 1285 | DSMZ Medium: 1285 (mediadive.medium:1285) | Aerobic: No
Composition
flowchart LR
CultureMech_004037["granulicella_paludicola_medium"]
ing0_Fructose["Fructose"]
CultureMech_004037 --> ing0_Fructose
CHEBI_28757["CHEBI:28757"]
ing0_Fructose -.-> CHEBI_28757
ing1_Yeast_extract["Yeast extract"]
CultureMech_004037 --> ing1_Yeast_extract
ing2_Casamino_acids["Casamino acids"]
CultureMech_004037 --> ing2_Casamino_acids
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004037 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004037 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_004037 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_004037 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_Agar["Agar"]
CultureMech_004037 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_alginic["alginic"]
CultureMech_004037 --> ing8_alginic
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Fructose | 0.5 G_PER_L |
CHEBI:28757
fructose
|
|
| 2 | Yeast extract | 0.05 G_PER_L | ||
| 3 | Casamino acids | 0.05 G_PER_L | ||
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.04 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 9 | alginic | variable VARIABLE | Extracted from recipe notes (pH buffer) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_1285_GRANULICELLA_PALUDICOLA_medium (CultureMech:004037)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1285 |
| 2026-02-03T11:18:55.818032 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1285 |
| 2026-03-13T06:19:24.345910Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004037 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: alginic |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.070076+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GRANULICELLA PALUDICOLA medium" to "granulicella_paludicola_medium" for consistent matching |
| 2026-05-16T09:03:01.063429+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-20T06:00:49.950042+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '4.0-5.0' -> {'min': 4.0, 'max': 5.0} |
| 2026-06-05T07:40:20.649342+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.728856+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:44.043459+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:42.846301+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 980b33958e6ff6c3b62d5a170840ccddeff8904bd87ae93a0caaf4e85fc3d2c9 |