Overview
- External ontology
- komodo.medium:1322 (source link unavailable) — METHYLOFERULA STELLATA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.0, 'max': 5.8}
- Notes
- pH buffer: H3PO4 | Source: KOMODO ModelSEED | ID: 1322 | DSMZ Medium: 1322 (mediadive.medium:1322) | Aerobic: No
Composition
flowchart LR
CultureMech_004078["methyloferula_stellata_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_004078 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_004078 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004078 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004078 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaCl["NaCl"]
CultureMech_004078 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Methanol["Methanol"]
CultureMech_004078 --> ing5_Methanol
CHEBI_17790["CHEBI:17790"]
ing5_Methanol -.-> CHEBI_17790
ing6_EDTA["EDTA"]
CultureMech_004078 --> ing6_EDTA
CHEBI_4735["CHEBI:4735"]
ing6_EDTA -.-> CHEBI_4735
ing7_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
CultureMech_004078 --> ing7_CuCl2_x_5_H2O
CHEBI_91245["CHEBI:91245"]
ing7_CuCl2_x_5_H2O -.-> CHEBI_91245
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004078 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004078 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004078 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004078 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_Na2MoO4["Na2MoO4"]
CultureMech_004078 --> ing12_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing12_Na2MoO4 -.-> CHEBI_75215
ing13_Na2_EDTA["Na2-EDTA"]
CultureMech_004078 --> ing13_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing13_Na2_EDTA -.-> CHEBI_64734
ing14_H3PO4["H3PO4"]
CultureMech_004078 --> ing14_H3PO4
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.02 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Methanol
CH4O 32.042 g/mol
|
7.92 G_PER_L |
CHEBI:17790
methanol
|
|
| 7 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 8 |
CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
|
0.1 G_PER_L |
CHEBI:91245
copper(II) chloride pentahydrate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
3.54 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 2.0, 1.54] |
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 14 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
2.06 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 15 | H3PO4 | variable VARIABLE | Extracted from recipe notes (pH buffer) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_1322_METHYLOFERULA_STELLATA_MEDIUM (CultureMech:004078)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1322 |
| 2026-02-03T11:19:10.422011 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1322 |
| 2026-03-13T03:09:19.651473Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:24.641021Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004078 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H3PO4 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.418229+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOFERULA STELLATA MEDIUM" to "methyloferula_stellata_medium" for consistent matching |
| 2026-05-16T09:03:01.182581+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-20T06:00:50.102943+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5.0-5.8' -> {'min': 5.0, 'max': 5.8} |
| 2026-06-05T07:40:20.841045+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.107621+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:43.116461+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 34fcf0de9b5a78cccb852a97f60769b646b15dca90ab8418f9546ae6ca232aef |