Overview
- External ontology
- komodo.medium:1457 (source link unavailable) — ALCALILIMNICOLA EHRLICHII MHLE-1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.8
- Notes
- Source: KOMODO ModelSEED | ID: 1457 | DSMZ Medium: 1457 (mediadive.medium:1457) | Aerobic: No
Composition
flowchart LR
CultureMech_004166["alcalilimnicola_ehrlichii_mhle_1_medium"]
ing0_Agar["Agar"]
CultureMech_004166 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_004166 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4["MgSO4"]
CultureMech_004166 --> ing2_MgSO4
CHEBI_32599["CHEBI:32599"]
ing2_MgSO4 -.-> CHEBI_32599
ing3_NaCl["NaCl"]
CultureMech_004166 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_004166 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KH2PO4["KH2PO4"]
CultureMech_004166 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Tungstate["Tungstate"]
CultureMech_004166 --> ing6_Tungstate
CHEBI_46502["CHEBI:46502"]
ing6_Tungstate -.-> CHEBI_46502
ing7_Na2CO3["Na2CO3"]
CultureMech_004166 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_NaHCO3["NaHCO3"]
CultureMech_004166 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na_acetate["Na-acetate"]
CultureMech_004166 --> ing9_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Na_acetate -.-> CHEBI_32954
ing10_Biotin["Biotin"]
CultureMech_004166 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_004166 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_004166 --> ing12_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_004166 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Riboflavin["Riboflavin"]
CultureMech_004166 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_004166 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_004166 --> ing16_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_D_Calcium_pantothenate -.-> CHEBI_31345
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_004166 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_004166 --> ing18_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_004166 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_HCl["HCl"]
CultureMech_004166 --> ing20_HCl
CHEBI_17883["CHEBI:17883"]
ing20_HCl -.-> CHEBI_17883
ing21_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_004166 --> ing21_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing21_FeCl2_x_4_H2O -.-> CHEBI_86249
ing22_ZnCl2["ZnCl2"]
CultureMech_004166 --> ing22_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing22_ZnCl2 -.-> CHEBI_49976
ing23_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004166 --> ing23_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing23_MnCl2_x_4_H2O -.-> CHEBI_86368
ing24_H3BO3["H3BO3"]
CultureMech_004166 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004166 --> ing25_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
ing26_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004166 --> ing26_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing26_CuCl2_x_2_H2O -.-> CHEBI_86318
ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004166 --> ing27_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004166 --> ing28_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 19.8807 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.201613 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.0504032 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
120.968 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.302419 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.16129 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Tungstate
O4W 247.836 g/mol
|
7.43514e-06 G_PER_L |
CHEBI:46502
tungstate
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
21.2 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
8.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.820338 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 17 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 19 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 21 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 22 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 23 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 24 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 26 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 27 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 28 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 29 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_1457_ALCALILIMNICOLA_EHRLICHII_MHLE-1_MEDIUM (CultureMech:004166)
- alcalilimnicola_ehrlichii_mhle_1_medium (CultureMech:000923)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1457 |
| 2026-02-03T11:19:18.968992 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1457 |
| 2026-03-13T06:19:25.577640Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004166 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.502412+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALCALILIMNICOLA EHRLICHII MHLE-1 MEDIUM" to "alcalilimnicola_ehrlichii_mhle_1_medium" for consistent matching |
| 2026-06-05T07:40:21.243691+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.245271+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:43.778359+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_1457_ALCALILIMNICOLA_EHRLICHII_MHLE-1_MEDIUM, alcalilimnicola_ehrlichii_mhle_1_medium |
| 2026-08-06T03:04:43.778410+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4c448336e673a5cade998ba53b2bfc0ec16ec65918d65970278ddcf472de6c97 |