thermoplasma_acidophilum_medium

CultureMech:004181 archaea COMPLEX LIQUID
Original name: THERMOPLASMA ACIDOPHILUM medium

Overview

External ontology
komodo.medium:158 (source link unavailable) — THERMOPLASMA ACIDOPHILUM medium
Applications
  • Microbial cultivation
pH
1.0
Notes
pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 158 | DSMZ Medium: 158 (mediadive.medium:158) | Aerobic: No

Composition

flowchart LR
    CultureMech_004181["thermoplasma_acidophilum_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_004181 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_004181 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004181 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004181 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_004181 --> ing4_Yeast_extract
    ing5_Glucose["Glucose"]
    CultureMech_004181 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_004181 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004181 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_004181 --> ing8_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing8_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004181 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004181 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004181 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_VOSO4_x_5_H2O["VOSO4 x 5 H2O"]
    CultureMech_004181 --> ing12_VOSO4_x_5_H2O
    CHEBI_132758["CHEBI:132758"]
    ing12_VOSO4_x_5_H2O -.-> CHEBI_132758
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_004181 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_H2SO4["H2SO4"]
    CultureMech_004181 --> ing14_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing14_H2SO4 -.-> CHEBI_26836

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.32 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.372 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.247 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.074 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Yeast extract 2 G_PER_L
6 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
7 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.93 G_PER_L CHEBI:86254
iron trichloride hexahydrate
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.18 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.45 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.005 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 VOSO4 x 5 H2O
5H2O.O4S.OV 253.081 g/mol
0.0038 G_PER_L CHEBI:132758
vanadyl sulfate pentahydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.002 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 158
2026-02-03T11:19:01.648673 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 158
2026-03-13T03:08:55.946691Z data-quality-cleanup-v1.0 Data quality fixes Added pH buffer: H2SO4
2026-03-13T06:19:25.681495Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004181
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:40.623555+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOPLASMA ACIDOPHILUM medium" to "thermoplasma_acidophilum_medium" for consistent matching
2026-05-16T09:03:01.480012+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-05-20T06:00:50.436689+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '1.0-2.0' -> {'min': 1.0, 'max': 2.0}
2026-06-05T07:40:21.297252+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:44.268514+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:22.580366+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3d517987eccfd77c826398fafc9ab6225c4695e69162f3c55192ec1f2d9dc7f5