Overview
- External ontology
- komodo.medium:221 (source link unavailable) — AZOSPIRILLUM medium
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: KOMODO ModelSEED | ID: 221 | DSMZ Medium: 221 (mediadive.medium:221) | Aerobic: No
Composition
flowchart LR
CultureMech_004576["azospirillum_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_004576 --> ing0_Yeast_extract
ing1_K2HPO4["K2HPO4"]
CultureMech_004576 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004576 --> ing2_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing2_FeSO4_x_7_H2O -.-> CHEBI_75836
ing3_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004576 --> ing3_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing3_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing4_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_004576 --> ing4_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing4_MnSO4_x_H2O -.-> CHEBI_86364
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004576 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_004576 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004576 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_004576 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_Biotin["Biotin"]
CultureMech_004576 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Agar["Agar"]
CultureMech_004576 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_Glucose["Glucose"]
CultureMech_004576 --> ing11_Glucose
CHEBI_17234["CHEBI:17234"]
ing11_Glucose -.-> CHEBI_17234
ing12_Na_malate["Na malate"]
CultureMech_004576 --> ing12_Na_malate
CHEBI_91260["CHEBI:91260"]
ing12_Na_malate -.-> CHEBI_91260
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.05 G_PER_L | ||
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.25 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 4 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.001 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 5 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.002 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0001 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 12 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 13 | Na malate | 5 G_PER_L |
CHEBI:91260
disodium malate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_221_AZOSPIRILLUM_medium (CultureMech:004576)
- azospirillum_medium (CultureMech:001325)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 221 |
| 2026-02-03T11:19:07.470085 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 221 |
| 2026-03-13T06:19:27.481287Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004576 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:42.708694+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AZOSPIRILLUM medium" to "azospirillum_medium" for consistent matching |
| 2026-06-05T07:40:21.775524+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.414732+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:45.831445+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_221_AZOSPIRILLUM_medium, azospirillum_medium |
| 2026-08-06T03:04:45.831524+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ea8b7a3069eef162caee8ffc0b7a19106222b3e676e9ceff6d04f9b17fa11b15 |