Overview
- External ontology
- komodo.medium:25 (source link unavailable) — RHODOPSEUDOMONAS GLOBIFORMIS medium
- Applications
-
- Microbial cultivation
- pH
- 4.9
- Notes
- Source: KOMODO ModelSEED | ID: 25 | DSMZ Medium: 25 (mediadive.medium:25) | Aerobic: No
Composition
flowchart LR
CultureMech_004663["rhodopseudomonas_globiformis_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_004663 --> ing0_Yeast_extract
ing1_Mannitol["Mannitol"]
CultureMech_004663 --> ing1_Mannitol
CHEBI_29864["CHEBI:29864"]
ing1_Mannitol -.-> CHEBI_29864
ing2_Na_gluconate["Na-gluconate"]
CultureMech_004663 --> ing2_Na_gluconate
CHEBI_84997["CHEBI:84997"]
ing2_Na_gluconate -.-> CHEBI_84997
ing3_KH2PO4["KH2PO4"]
CultureMech_004663 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004663 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_NH4Cl["NH4Cl"]
CultureMech_004663 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_NaCl["NaCl"]
CultureMech_004663 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004663 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Fe_III__citrate["Fe(III) citrate"]
CultureMech_004663 --> ing8_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing8_Fe_III__citrate -.-> CHEBI_144421
ing9_Biotin["Biotin"]
CultureMech_004663 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_004663 --> ing10_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing10_p_Aminobenzoic_acid -.-> CHEBI_30753
ing11_Sodium_thiosulfate_pentahydrate["Sodium thiosulfate pentahydrate"]
CultureMech_004663 --> ing11_Sodium_thiosulfate_pentahydrate
CHEBI_32150["CHEBI:32150"]
ing11_Sodium_thiosulfate_pentahydrate -.-> CHEBI_32150
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004663 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004663 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_004663 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004663 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004663 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004663 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004663 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.25 G_PER_L | ||
| 2 |
Mannitol
C6H14O6 182.172 g/mol
|
1.5 G_PER_L |
CHEBI:29864
mannitol
|
|
| 3 |
Na-gluconate
C6H11O7.Na 218.137 g/mol
|
0.5 G_PER_L |
CHEBI:84997
sodium gluconate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.005 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0001 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 12 |
Sodium thiosulfate pentahydrate
5H2O.2Na.O3S2 248.186 g/mol
|
0.2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_25_RHODOPSEUDOMONAS_GLOBIFORMIS_medium (CultureMech:004663)
- rhodopseudomonas_globiformis_medium (CultureMech:001358)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 25 |
| 2026-02-03T11:18:55.428980 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 25 |
| 2026-03-13T06:19:27.938876Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004663 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:43.246257+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RHODOPSEUDOMONAS GLOBIFORMIS medium" to "rhodopseudomonas_globiformis_medium" for consistent matching |
| 2026-06-05T07:40:21.932910+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.459031+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:46.258615+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_25_RHODOPSEUDOMONAS_GLOBIFORMIS_medium, rhodopseudomonas_globiformis_medium |
| 2026-08-06T03:04:46.258653+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 9f432cbeb889a63765e7448e61f954c5eb946ba96cc0e3a90bc0c2a624b22b20 |