Overview
- External ontology
- komodo.medium:320 (source link unavailable) — CLOSTRIDIUM CELLULOVORANS medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'clostridium_cellulovorans_medium_replace_rumen_fluid_clarified_with_sludge_fluid_medium_119', 'source': 'KOMODO', 'source_id': 'komodo.medium:320_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 320 | DSMZ Medium: 320 (mediadive.medium:320) | Aerobic: Yes
Composition
flowchart LR
CultureMech_005010["clostridium_cellulovorans_medium"]
ing0_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_005010 --> ing0_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing1_NH4Cl["NH4Cl"]
CultureMech_005010 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KCl["KCl"]
CultureMech_005010 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005010 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Trypticase_peptone["Trypticase peptone"]
CultureMech_005010 --> ing4_Trypticase_peptone
ing5_Yeast_extract["Yeast extract"]
CultureMech_005010 --> ing5_Yeast_extract
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_005010 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005010 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_Na2CO3["Na2CO3"]
CultureMech_005010 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Cellobiose["Cellobiose"]
CultureMech_005010 --> ing9_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing9_Cellobiose -.-> CHEBI_17057
ing10_Cellulose["Cellulose"]
CultureMech_005010 --> ing10_Cellulose
CHEBI_18246["CHEBI:18246"]
ing10_Cellulose -.-> CHEBI_18246
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005010 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_HCl["HCl"]
CultureMech_005010 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_005010 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_005010 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005010 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_005010 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005010 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005010 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005010 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005010 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_Sludge["Sludge"]
CultureMech_005010 --> ing21_Sludge
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Trypticase peptone | 0.5 G_PER_L | ||
| 6 | Yeast extract | 4.5 G_PER_L | [Merged 2 duplicates: 0.5, 4.0] | |
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.15 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Cellobiose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 11 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
10 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Optional ingredient (MN 301) |
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 | Sludge | 1000 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_320_CLOSTRIDIUM_CELLULOVORANS_medium (CultureMech:005010)
- clostridium_cellulovorans_medium (CultureMech:001422)
- clostridium_cellulovorans_medium_replace_rumen_fluid_clarified_with_sludge_fluid_medium_119 (CultureMech:005009)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 320 |
| 2026-02-03T11:19:08.059935 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 320 |
| 2026-03-13T03:09:13.278764Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:29.465475Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005010 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.041046+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM CELLULOVORANS medium" to "clostridium_cellulovorans_medium" for consistent matching |
| 2026-06-05T07:40:22.451715+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.585756+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:47.401931+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_320_CLOSTRIDIUM_CELLULOVORANS_medium, clostridium_cellulovorans_medium, clostridium_cellulovorans_medium_replace_rumen_fluid_clarified_with_sludge_fluid_medium_119 |
| 2026-08-06T03:04:47.402003+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint c29b33a7ca9fce1d33c34fe753e8f39689a611150477471f42996e3db6fb69ff |