Overview
- External ontology
- komodo.medium:328 (source link unavailable) — PYG medium WITH VOLATILE FATTY ACIDS
- Applications
-
- Microbial cultivation
- pH
- 6.9
- Notes
- Source: KOMODO ModelSEED | ID: 328 | DSMZ Medium: 328 (mediadive.medium:328) | Aerobic: Yes Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_005021["pyg_medium_with_volatile_fatty_acids"]
ing0_Beef_extract["Beef extract"]
CultureMech_005021 --> ing0_Beef_extract
ing1_Trypticase["Trypticase"]
CultureMech_005021 --> ing1_Trypticase
ing2_Yeast_extract["Yeast extract"]
CultureMech_005021 --> ing2_Yeast_extract
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_005021 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Glucose["Glucose"]
CultureMech_005021 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Resazurin["Resazurin"]
CultureMech_005021 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Haemin["Haemin"]
CultureMech_005021 --> ing6_Haemin
CHEBI_50385["CHEBI:50385"]
ing6_Haemin -.-> CHEBI_50385
ing7_Vitamin_K1["Vitamin K1"]
CultureMech_005021 --> ing7_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing7_Vitamin_K1 -.-> CHEBI_18067
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005021 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005021 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005021 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_K2HPO4["K2HPO4"]
CultureMech_005021 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
ing12_KH2PO4["KH2PO4"]
CultureMech_005021 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_NaHCO3["NaHCO3"]
CultureMech_005021 --> ing13_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing13_NaHCO3 -.-> CHEBI_32139
ing14_NaCl["NaCl"]
CultureMech_005021 --> ing14_NaCl
CHEBI_26710["CHEBI:26710"]
ing14_NaCl -.-> CHEBI_26710
ing15_Acetic_acid["Acetic acid"]
CultureMech_005021 --> ing15_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing15_Acetic_acid -.-> CHEBI_15366
ing16_Propionic_acid["Propionic acid"]
CultureMech_005021 --> ing16_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing16_Propionic_acid -.-> CHEBI_30768
ing17_n_Butyric_acid["n-Butyric acid"]
CultureMech_005021 --> ing17_n_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing17_n_Butyric_acid -.-> CHEBI_30772
ing18_n_Valeric_acid["n-Valeric acid"]
CultureMech_005021 --> ing18_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing18_n_Valeric_acid -.-> CHEBI_17418
ing19_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_005021 --> ing19_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing19_iso_Valeric_acid -.-> CHEBI_28484
ing20_iso_Butyric_acid["iso-Butyric acid"]
CultureMech_005021 --> ing20_iso_Butyric_acid
CHEBI_16135["CHEBI:16135"]
ing20_iso_Butyric_acid -.-> CHEBI_16135
ing21_Tryptone["Tryptone"]
CultureMech_005021 --> ing21_Tryptone
ing22_Yeast_extract["Yeast extract"]
CultureMech_005021 --> ing22_Yeast_extract
ing23_Sodium_chloride["Sodium chloride"]
CultureMech_005021 --> ing23_Sodium_chloride
ing23_Sodium_chloride -.-> CHEBI_26710
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Beef extract | 5 G_PER_L | ||
| 2 | Trypticase | 5 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.005 G_PER_L |
CHEBI:50385
hemin
|
|
| 8 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.001 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.48 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 15 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 16 |
Acetic acid
C2H4O2 60.052 g/mol
|
17 G_PER_L |
CHEBI:15366
acetic acid
|
|
| 17 |
Propionic acid
C3H6O2 74.079 g/mol
|
6 G_PER_L |
CHEBI:30768
propionic acid
|
|
| 18 |
n-Butyric acid
C4H8O2 88.106 g/mol
|
4 G_PER_L |
CHEBI:30772
butyric acid
|
|
| 19 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 20 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 21 |
iso-Butyric acid
C4H8O2 88.106 g/mol
|
1 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 22 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 23 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 24 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_328_PYG_medium_WITH_VOLATILE_FATTY_ACIDS (CultureMech:005021)
- pyg_medium_with_volatile_fatty_acids (CultureMech:001428)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 328 |
| 2026-02-03T11:19:08.890455 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 328 |
| 2026-03-13T06:19:29.580443Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005021 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.170376+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG medium WITH VOLATILE FATTY ACIDS" to "pyg_medium_with_volatile_fatty_acids" for consistent matching |
| 2026-06-05T07:40:22.480054+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.596418+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/1/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-09-22T00:08:40.662178+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_328_PYG_medium_WITH_VOLATILE_FATTY_ACIDS, pyg_medium_with_volatile_fatty_acids |
| 2026-09-22T00:08:40.662221+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 93a64c518633110e47c33ce1dc1e7e0d538558046c4ae61d87ba69ae8b0afb58 |