Overview
- External ontology
- komodo.medium:39 (source link unavailable) — PFENNIG'S medium I WITH Yeast extract
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 39 | DSMZ Medium: 39 (mediadive.medium:39) | Aerobic: No
Composition
flowchart LR
CultureMech_005189["pfennigs_medium_i_with_yeast_extract"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_005189 --> ing0_Yeast_extract
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005189 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005189 --> ing2_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing2_Na2S_x_9_H2O -.-> CHEBI_76209
ing3_NaHCO3["NaHCO3"]
CultureMech_005189 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Resazurin["Resazurin"]
CultureMech_005189 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Ammonium_chloride["Ammonium chloride"]
CultureMech_005189 --> ing5_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing5_Ammonium_chloride -.-> CHEBI_31206
ing6_Ammonium_acetate["Ammonium acetate"]
CultureMech_005189 --> ing6_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing6_Ammonium_acetate -.-> CHEBI_62947
ing7_Pyruvic_acid_sodium_salt["Pyruvic acid sodium salt"]
CultureMech_005189 --> ing7_Pyruvic_acid_sodium_salt
CHEBI_50144["CHEBI:50144"]
ing7_Pyruvic_acid_sodium_salt -.-> CHEBI_50144
ing8_Dextrose["Dextrose"]
CultureMech_005189 --> ing8_Dextrose
CHEBI_17634["CHEBI:17634"]
ing8_Dextrose -.-> CHEBI_17634
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005189 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_KCl["KCl"]
CultureMech_005189 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_KH2PO4["KH2PO4"]
CultureMech_005189 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_005189 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1.043478 G_PER_L | [Merged 2 duplicates: 0.5, 0.543478] | |
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.543478 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
44.8148 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
[Merged 2 duplicates: 14.8148, 30.0] |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0111111 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
13.4615 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
9.61539 G_PER_L |
CHEBI:62947
ammonium acetate
|
|
| 8 |
Pyruvic acid sodium salt
C3H3O3.Na 110.044 g/mol
|
9.61539 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 9 |
Dextrose
C6H12O6 180.156 g/mol
|
9.61539 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
19.2308 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
13.4615 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
13.4615 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- pfennigs_medium_i_with_yeast_extract (normalized source unresolved)
- pfennigs_medium_i_with_yeast_extract (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 39 |
| 2026-02-03T11:19:07.560676 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 39 |
| 2026-03-13T03:09:12.273689Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:30.956865Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005189 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:46.781727+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PFENNIG'S medium I WITH Yeast extract" to "pfennigs_medium_i_with_yeast_extract" for consistent matching |
| 2026-06-05T07:41:41.596491+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.130315+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.777189+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: pfennigs_medium_i_with_yeast_extract, pfennigs_medium_i_with_yeast_extract |
| 2026-08-06T03:05:52.777242+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint f94b1fde47e70358ce165272fa4283abda3bd6079cb5590fa25fab0e39725b20 |