Overview
- External ontology
- komodo.medium:403 (source link unavailable) — BSK-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 403 | DSMZ Medium: 403 (mediadive.medium:403) | Aerobic: No Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_005218["bsk_medium"]
ing0_CMRL_1066["CMRL 1066"]
CultureMech_005218 --> ing0_CMRL_1066
ing1_Proteose_peptone_no__2["Proteose peptone no. 2"]
CultureMech_005218 --> ing1_Proteose_peptone_no__2
ing2_Tryptone_peptone["Tryptone peptone"]
CultureMech_005218 --> ing2_Tryptone_peptone
ing3_Proteose_yeastolate["Proteose yeastolate"]
CultureMech_005218 --> ing3_Proteose_yeastolate
ing4_HEPES_buffer["HEPES buffer"]
CultureMech_005218 --> ing4_HEPES_buffer
CHEBI_46756["CHEBI:46756"]
ing4_HEPES_buffer -.-> CHEBI_46756
ing5_Sodium_citrate["Sodium citrate"]
CultureMech_005218 --> ing5_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Sodium_citrate -.-> CHEBI_53258
ing6_Glucose["Glucose"]
CultureMech_005218 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Na_pyruvate["Na-pyruvate"]
CultureMech_005218 --> ing7_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Na_pyruvate -.-> CHEBI_50144
ing8_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_005218 --> ing8_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing8_N_Acetylglucosamine -.-> CHEBI_59640
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005218 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_L_Glutamine["L-Glutamine"]
CultureMech_005218 --> ing10_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing10_L_Glutamine -.-> CHEBI_18050
ing11_Double_distilled_water["Double distilled water"]
CultureMech_005218 --> ing11_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing11_Double_distilled_water -.-> CHEBI_15377
ing12_Gelatine["Gelatine"]
CultureMech_005218 --> ing12_Gelatine
CHEBI_5291["CHEBI:5291"]
ing12_Gelatine -.-> CHEBI_5291
ing13_Tryptone["Tryptone"]
CultureMech_005218 --> ing13_Tryptone
ing14_Yeast_extract["Yeast extract"]
CultureMech_005218 --> ing14_Yeast_extract
ing15_Sodium_chloride["Sodium chloride"]
CultureMech_005218 --> ing15_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing15_Sodium_chloride -.-> CHEBI_26710
ing16_Rabbit_serum["Rabbit serum"]
CultureMech_005218 --> ing16_Rabbit_serum
ing17_NaOH["NaOH"]
CultureMech_005218 --> ing17_NaOH
CHEBI_32145["CHEBI:32145"]
ing17_NaOH -.-> CHEBI_32145
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | CMRL 1066 | 100 G_PER_L | ||
| 2 | Proteose peptone no. 2 | 5 G_PER_L | ||
| 3 | Tryptone peptone | 1 G_PER_L | ||
| 4 | Proteose yeastolate | 1 G_PER_L | ||
| 5 | HEPES buffer | 6 G_PER_L |
CHEBI:46756
HEPES
|
|
| 6 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.7 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 9 | N-Acetylglucosamine | 0.4 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
0.1 G_PER_L |
CHEBI:18050
L-glutamine
|
|
| 12 |
Double distilled water
H2O 18.015 g/mol
|
1243.0 G_PER_L |
CHEBI:15377
water
|
[Merged 3 duplicates: 900.0, 200.0, 143.0] |
| 13 | Gelatine | 70 G_PER_L |
CHEBI:5291
gelatin
|
|
| 14 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 15 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 16 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 17 | Rabbit serum | 86 G_PER_L | ||
| 18 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_403_BSK-MEDIUM (CultureMech:005218)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 403 |
| 2026-02-03T11:19:15.646993 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 403 |
| 2026-03-13T03:09:33.578816Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s); Added pH buffer: NaOH |
| 2026-03-13T06:19:31.121187Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005218 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:46.973678+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSK-MEDIUM" to "bsk_medium" for consistent matching |
| 2026-05-16T09:03:02.579823+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:23.080159+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.811162+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:48.754532+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0411c62830ad68d6ee5f0f8128ba55cbf1819cb78e9c0a105e1502d64ced3142 |