Overview
- External ontology
- komodo.medium:40 (source link unavailable) — PFENNIG'S MEDIUM II WITH SALT
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: KOMODO ModelSEED | ID: 40 | DSMZ Medium: 40 (mediadive.medium:40) | Aerobic: No
Composition
flowchart LR
CultureMech_005231["pfennigs_medium_ii_with_salt"]
ing0_NaCl["NaCl"]
CultureMech_005231 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005231 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_Yeast_extract["Yeast extract"]
CultureMech_005231 --> ing2_Yeast_extract
ing3_NaHCO3["NaHCO3"]
CultureMech_005231 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Resazurin["Resazurin"]
CultureMech_005231 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Ammonium_chloride["Ammonium chloride"]
CultureMech_005231 --> ing5_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing5_Ammonium_chloride -.-> CHEBI_31206
ing6_Ammonium_acetate["Ammonium acetate"]
CultureMech_005231 --> ing6_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing6_Ammonium_acetate -.-> CHEBI_62947
ing7_Pyruvic_acid_sodium_salt["Pyruvic acid sodium salt"]
CultureMech_005231 --> ing7_Pyruvic_acid_sodium_salt
CHEBI_50144["CHEBI:50144"]
ing7_Pyruvic_acid_sodium_salt -.-> CHEBI_50144
ing8_Dextrose["Dextrose"]
CultureMech_005231 --> ing8_Dextrose
CHEBI_17634["CHEBI:17634"]
ing8_Dextrose -.-> CHEBI_17634
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005231 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_KCl["KCl"]
CultureMech_005231 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_KH2PO4["KH2PO4"]
CultureMech_005231 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_005231 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005231 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Na2_EDTA["Na2-EDTA"]
CultureMech_005231 --> ing14_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing14_Na2_EDTA -.-> CHEBI_64734
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005231 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005231 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
CultureMech_005231 --> ing17_MnCl2_x_2_H2O
CHEBI_131395["CHEBI:131395"]
ing17_MnCl2_x_2_H2O -.-> CHEBI_131395
ing18_ZnCl2["ZnCl2"]
CultureMech_005231 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005231 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005231 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_H3BO3["H3BO3"]
CultureMech_005231 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005231 --> ing22_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.086956 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.543478, 0.543478] |
| 3 | Yeast extract | 1.086956 G_PER_L | [Merged 2 duplicates: 0.543478, 0.543478] | |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
60.0 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
[Merged 2 duplicates: 30.0, 30.0] |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0161111 G_PER_L |
CHEBI:8806
Resazurin
|
[Merged 2 duplicates: 0.005, 0.0111111] |
| 6 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
27.4615 G_PER_L |
CHEBI:31206
ammonium chloride
|
[Merged 2 duplicates: 14.0, 13.4615] |
| 7 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
19.615389999999998 G_PER_L |
CHEBI:62947
ammonium acetate
|
[Merged 2 duplicates: 10.0, 9.61539] |
| 8 |
Pyruvic acid sodium salt
C3H3O3.Na 110.044 g/mol
|
19.615389999999998 G_PER_L |
CHEBI:50144
sodium pyruvate
|
[Merged 2 duplicates: 10.0, 9.61539] |
| 9 |
Dextrose
C6H12O6 180.156 g/mol
|
19.615389999999998 G_PER_L |
CHEBI:17634
D-glucose
|
[Merged 2 duplicates: 10.0, 9.61539] |
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
39.2308 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 20.0, 19.2308] |
| 11 |
KCl
Cl.K 74.551 g/mol
|
27.4615 G_PER_L |
CHEBI:32588
potassium chloride
|
[Merged 2 duplicates: 14.0, 13.4615] |
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
27.4615 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
[Merged 2 duplicates: 14.0, 13.4615] |
| 13 | Vitamin B12 | 0.2 G_PER_L |
CHEBI:176843
vitamin B12
|
[Merged 2 duplicates: 0.1, 0.1] |
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
44.8148 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
[Merged 2 duplicates: 14.8148, 30.0] |
| 15 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
3 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.05 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
|
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.042 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.018 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_40_PFENNIG_S_MEDIUM_II_WITH_SALT (CultureMech:005231)
- pfennigs_medium_ii_with_salt (CultureMech:001518)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 40 |
| 2026-02-03T11:18:59.954821 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 40 |
| 2026-03-13T03:08:51.783466Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 13 duplicate ingredient(s) |
| 2026-03-13T06:19:31.185427Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005231 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:47.047834+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PFENNIG'S MEDIUM II WITH SALT" to "pfennigs_medium_ii_with_salt" for consistent matching |
| 2026-06-05T07:40:23.100515+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.819024+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:48.796803+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_40_PFENNIG_S_MEDIUM_II_WITH_SALT, pfennigs_medium_ii_with_salt |
| 2026-08-06T03:04:48.796851+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 8ecb7e9f1c6ebb7b61e04eeb88c5dfb0897daecd9f3a43d4f6ae0bc5eb7ce873 |