Overview
- External ontology
- komodo.medium:428 (source link unavailable) — HETEROTROPHIC medium H3P
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 428 | DSMZ Medium: 428 (mediadive.medium:428) | Aerobic: No
Composition
flowchart LR
CultureMech_005254["heterotrophic_medium_h3p"]
ing0_KH2PO4["KH2PO4"]
CultureMech_005254 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_005254 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_NH4Cl["NH4Cl"]
CultureMech_005254 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005254 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005254 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005254 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_NaVO3_x_H2O["NaVO3 x H2O"]
CultureMech_005254 --> ing6_NaVO3_x_H2O
CHEBI_132095["CHEBI:132095"]
ing6_NaVO3_x_H2O -.-> CHEBI_132095
ing7_Agar["Agar"]
CultureMech_005254 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_005254 --> ing8_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing8_Ferric_ammonium_citrate -.-> CHEBI_31604
ing9_Yeast_extract["Yeast extract"]
CultureMech_005254 --> ing9_Yeast_extract
ing10_Na_acetate["Na-acetate"]
CultureMech_005254 --> ing10_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Na_acetate -.-> CHEBI_32954
ing11_Disodium_succinate["Disodium succinate"]
CultureMech_005254 --> ing11_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing11_Disodium_succinate -.-> CHEBI_63675
ing12_DL_Malate["DL-Malate"]
CultureMech_005254 --> ing12_DL_Malate
CHEBI_15595["CHEBI:15595"]
ing12_DL_Malate -.-> CHEBI_15595
ing13_Na_lactate["Na-lactate"]
CultureMech_005254 --> ing13_Na_lactate
CHEBI_75228["CHEBI:75228"]
ing13_Na_lactate -.-> CHEBI_75228
ing14_Na_pyruvate["Na-pyruvate"]
CultureMech_005254 --> ing14_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing14_Na_pyruvate -.-> CHEBI_50144
ing15_D_Mannitol["D-Mannitol"]
CultureMech_005254 --> ing15_D_Mannitol
CHEBI_16899["CHEBI:16899"]
ing15_D_Mannitol -.-> CHEBI_16899
ing16_D_Glucose["D-Glucose"]
CultureMech_005254 --> ing16_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing16_D_Glucose -.-> CHEBI_17634
ing17_Riboflavin["Riboflavin"]
CultureMech_005254 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_005254 --> ing18_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing18_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_005254 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_005254 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_005254 --> ing21_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_pantothenate -.-> CHEBI_31345
ing22_Biotin["Biotin"]
CultureMech_005254 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_005254 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_005254 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005254 --> ing25_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing26_H3BO3["H3BO3"]
CultureMech_005254 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005254 --> ing27_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing27_CoCl2_x_6_H2O -.-> CHEBI_53503
ing28_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005254 --> ing28_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing28_CuCl2_x_2_H2O -.-> CHEBI_86318
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005254 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_005254 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
46 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
58 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.16959 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.584795 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0116959 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.035847949999999996 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
[Merged 2 duplicates: 0.00584795, 0.03] |
| 7 |
NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
|
0.00584795 G_PER_L |
CHEBI:132095
sodium metavanadate hydrate
|
|
| 8 | Agar | 23.3918 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 9 | Ferric ammonium citrate | 2.5 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 10 | Yeast extract | 33.3333 G_PER_L | ||
| 11 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
33.3333 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 12 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
33.3333 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 13 |
DL-Malate
C4H4O5 132.071 g/mol
|
33.3333 G_PER_L |
CHEBI:15595
malate(2-)
|
|
| 14 |
Na-lactate
C3H5O3.Na 112.06 g/mol
|
20 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 15 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
20 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 16 |
D-Mannitol
C6H14O6 182.172 g/mol
|
20 G_PER_L |
CHEBI:16899
D-mannitol
|
|
| 17 |
D-Glucose
C6H12O6 180.156 g/mol
|
40 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.1 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.5 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.5 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 22 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 29 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_428_HETEROTROPHIC_medium_H3P (CultureMech:005254)
- heterotrophic_medium_h3p (CultureMech:001532)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 428 |
| 2026-02-03T11:18:54.909530 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 428 |
| 2026-03-13T03:08:39.321835Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:31.337396Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005254 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:47.210300+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HETEROTROPHIC medium H3P" to "heterotrophic_medium_h3p" for consistent matching |
| 2026-06-05T07:40:23.209854+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.847966+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:49.057962+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_428_HETEROTROPHIC_medium_H3P, heterotrophic_medium_h3p |
| 2026-08-06T03:04:49.058013+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 6b8943ee985d35158080198e11aea1ce63103db6da6983317669852a6b24beac |