Overview
- External ontology
- komodo.medium:470 (source link unavailable) — PSEUDOMONAS HALOPHILA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 470 | DSMZ Medium: 470 (mediadive.medium:470) | Aerobic: No
Composition
flowchart LR
CultureMech_005583["pseudomonas_halophila_medium"]
ing0_NaCl["NaCl"]
CultureMech_005583 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005583 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NH4Cl["NH4Cl"]
CultureMech_005583 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Glycerol["Glycerol"]
CultureMech_005583 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_KH2PO4["KH2PO4"]
CultureMech_005583 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_HCl["HCl"]
CultureMech_005583 --> ing5_HCl
CHEBI_17883["CHEBI:17883"]
ing5_HCl -.-> CHEBI_17883
ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_005583 --> ing6_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
ing7_ZnCl2["ZnCl2"]
CultureMech_005583 --> ing7_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing7_ZnCl2 -.-> CHEBI_49976
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_005583 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_005583 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_005583 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_005583 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005583 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005583 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_Biotin["Biotin"]
CultureMech_005583 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_005583 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_005583 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_005583 --> ing17_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing18_Riboflavin["Riboflavin"]
CultureMech_005583 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_005583 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_005583 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_005583 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_005583 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_NaOH["NaOH"]
CultureMech_005583 --> ing23_NaOH
CHEBI_32145["CHEBI:32145"]
ing23_NaOH -.-> CHEBI_32145
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
51.9423 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
43.7292 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.10988 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
5.54939 G_PER_L |
CHEBI:17754
glycerol
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 7 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 8 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.001 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pseudomonas_halophila_medium (CultureMech:005583)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 470 |
| 2026-02-03T11:19:11.940284 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 470 |
| 2026-03-13T03:09:24.080184Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:34.293201Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005583 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:50.505925+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSEUDOMONAS HALOPHILA MEDIUM" to "pseudomonas_halophila_medium" for consistent matching |
| 2026-05-16T09:04:29.581082+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:42.731844+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.472074+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.639349+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6c062093a591c7b034f1fc85e968e13e00075feed37b80a8435c91ab5edb1e26 |