Overview
- External ontology
- komodo.medium:534 (source link unavailable) — MODIFIED SME MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.6
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 534 | DSMZ Medium: 534 (mediadive.medium:534) | Aerobic: No
Composition
flowchart LR
CultureMech_005912["modified_sme_medium"]
ing0_NaCl["NaCl"]
CultureMech_005912 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005912 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_005912 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_005912 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaBr["NaBr"]
CultureMech_005912 --> ing4_NaBr
CHEBI_63004["CHEBI:63004"]
ing4_NaBr -.-> CHEBI_63004
ing5_NH4Cl["NH4Cl"]
CultureMech_005912 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_K2HPO4["K2HPO4"]
CultureMech_005912 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005912 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
CultureMech_005912 --> ing8__NH4_2Ni_SO4_2
CHEBI_86149["CHEBI:86149"]
ing8__NH4_2Ni_SO4_2 -.-> CHEBI_86149
ing9_Na2WO4["Na2WO4"]
CultureMech_005912 --> ing9_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing9_Na2WO4 -.-> CHEBI_63940
ing10_Na2SeO4["Na2SeO4"]
CultureMech_005912 --> ing10_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing10_Na2SeO4 -.-> CHEBI_77775
ing11_NaHCO3["NaHCO3"]
CultureMech_005912 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_005912 --> ing12_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing12_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_005912 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_005912 --> ing14_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_x_H2O -.-> CHEBI_86364
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005912 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_005912 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_005912 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_005912 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_005912 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_H3BO3["H3BO3"]
CultureMech_005912 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_005912 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_005912 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_005912 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_H2SO4["H2SO4"]
CultureMech_005912 --> ing24_H2SO4
CHEBI_26836["CHEBI:26836"]
ing24_H2SO4 -.-> CHEBI_26836
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
31.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 30.0, 1.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 7.0, 3.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.65 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.15 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.15 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.5, 0.1] |
| 9 |
(NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 10 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.01 G_PER_L |
CHEBI:63940
sodium tungstate
|
|
| 11 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.01 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
66.6667 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
66.6667 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_534_MODIFIED_SME_MEDIUM (CultureMech:005912)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 534 |
| 2026-02-03T11:19:09.182649 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 534 |
| 2026-03-13T03:09:16.439055Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s); Added pH buffer: H2SO4 |
| 2026-03-13T06:19:37.219100Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005912 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:53.940301+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED SME MEDIUM" to "modified_sme_medium" for consistent matching |
| 2026-05-16T09:03:03.368056+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-20T06:00:52.527811+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.5-6.8' -> {'min': 6.5, 'max': 6.8} |
| 2026-06-05T07:40:24.437501+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.266315+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:45.250365+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:55.229169+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 33070020dd3304a3f8c67e5a80420a056a52647a5afb831592f7c73ca458463f |