Overview
- External ontology
- komodo.medium:621 (source link unavailable) — PYGV AGAR
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- pH buffer: KOH | Source: KOMODO ModelSEED | ID: 621 | DSMZ Medium: 621 (mediadive.medium:621) | Aerobic: No
Composition
flowchart LR
CultureMech_006117["pygv_agar"]
ing0_Peptone["Peptone"]
CultureMech_006117 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_006117 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_006117 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Glucose["Glucose"]
CultureMech_006117 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_006117 --> ing4_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006117 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006117 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006117 --> ing7_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006117 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_Biotin["Biotin"]
CultureMech_006117 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_006117 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_006117 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Riboflavine["Riboflavine"]
CultureMech_006117 --> ing12_Riboflavine
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavine -.-> CHEBI_17015
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006117 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Nicotinamide["Nicotinamide"]
CultureMech_006117 --> ing14_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing14_Nicotinamide -.-> CHEBI_17154
ing15_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_006117 --> ing15_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_D_Calcium_pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_006117 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006117 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_KOH["KOH"]
CultureMech_006117 --> ing18_KOH
CHEBI_32035["CHEBI:32035"]
ing18_KOH -.-> CHEBI_32035
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.25 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10.5263 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
31.2632 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.51579 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0133368 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.104211 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.02 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Riboflavine
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 16 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
pH adjustment (extracted from notes) |
Solutions
Metall salt sol. 44
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_621_PYGV_AGAR (CultureMech:006117)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 621 |
| 2026-02-03T11:19:06.490791 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 621 |
| 2026-03-09T08:04:39.752123+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:09.635558Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: KOH |
| 2026-03-13T06:19:38.399727Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006117 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:55.312480+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYGV AGAR" to "pygv_agar" for consistent matching |
| 2026-05-16T09:03:03.710767+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:24.997216+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:56.258146+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2074da8221613bdb80164513717570994c2d4c45aeb8fd8856e889775bd92f9f |