Overview
- External ontology
- komodo.medium:651 (source link unavailable) — R 8 A H medium
- Applications
-
- Microbial cultivation
- pH
- 6.9
- Notes
- Source: KOMODO ModelSEED | ID: 651 | DSMZ Medium: 651 (mediadive.medium:651) | Aerobic: No
Composition
flowchart LR
CultureMech_006228["r_8_a_h_medium"]
ing0_Malic_acid["Malic acid"]
CultureMech_006228 --> ing0_Malic_acid
CHEBI_6650["CHEBI:6650"]
ing0_Malic_acid -.-> CHEBI_6650
ing1_Yeast_extract["Yeast extract"]
CultureMech_006228 --> ing1_Yeast_extract
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_006228 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006228 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006228 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Fe_III__citrate["Fe(III) citrate"]
CultureMech_006228 --> ing5_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing5_Fe_III__citrate -.-> CHEBI_144421
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_006228 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_KH2PO4["KH2PO4"]
CultureMech_006228 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_K2HPO4["K2HPO4"]
CultureMech_006228 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006228 --> ing9_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing9_MnSO4_x_H2O -.-> CHEBI_86364
ing10_H3BO3["H3BO3"]
CultureMech_006228 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006228 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_006228 --> ing12__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing12__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing13_ZnSO4["ZnSO4"]
CultureMech_006228 --> ing13_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing13_ZnSO4 -.-> CHEBI_35176
ing14_EDTA["EDTA"]
CultureMech_006228 --> ing14_EDTA
CHEBI_4735["CHEBI:4735"]
ing14_EDTA -.-> CHEBI_4735
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_006228 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Nicotinamide["Nicotinamide"]
CultureMech_006228 --> ing16_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing16_Nicotinamide -.-> CHEBI_17154
ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006228 --> ing17_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing18_Biotin["Biotin"]
CultureMech_006228 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Malic acid
C4H6O5 134.087 g/mol
|
2.5 G_PER_L |
CHEBI:6650
malic acid
|
|
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.27 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.07, 0.2] |
| 6 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
3.01 G_PER_L |
CHEBI:144421
iron(III) citrate
|
[Merged 2 duplicates: 0.01, 3.0] |
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.02 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.02 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.02 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 14 |
ZnSO4
O4S.Zn 161.453 g/mol
|
0.01 G_PER_L |
CHEBI:35176
zinc sulfate
|
|
| 15 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 17 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.2 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 18 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.4 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.008 G_PER_L |
CHEBI:15956
biotin
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_651_R_8_A_H_medium (CultureMech:006228)
- r_8_a_h_medium (CultureMech:001790)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 651 |
| 2026-02-03T11:19:14.850659 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 651 |
| 2026-03-13T03:09:31.533521Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:39.144265Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006228 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.201346+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R 8 A H medium" to "r_8_a_h_medium" for consistent matching |
| 2026-06-05T07:40:25.207415+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.474634+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:56.836398+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_651_R_8_A_H_medium, r_8_a_h_medium |
| 2026-08-06T03:04:56.836441+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 1df3df53ee12c6e9ab1e4ed8bd848c6c7876984fb5e3f0ed236bffbf1a4ab6f4 |