columbia_blood_medium

CultureMech:006311 bacterial COMPLEX SOLID_AGAR
Original name: COLUMBIA BLOOD medium

Overview

External ontology
komodo.medium:693 (source link unavailable) — COLUMBIA BLOOD medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_693_modified_for_dsm_13661', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_13661', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_13760', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_13760', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_18826', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_18826', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_19736', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_19736', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_20600', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_20600', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_21031', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_21031', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_21825', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_21825', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_21843', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_21843', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_21845', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_21845', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_22221', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_22221', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_22886', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_22886', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_22909', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_22909', 'original_category': 'bacterial'}
  • {'name': 'medium_693_modified_for_dsm_23385', 'source': 'KOMODO', 'source_id': 'komodo.medium:693_23385', 'original_category': 'bacterial'}
Notes
Source: KOMODO ModelSEED | ID: 693 | DSMZ Medium: 693 (mediadive.medium:693) | Aerobic: No Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_006311["columbia_blood_medium"]
    ing0_Columbia_agar_base["Columbia agar base"]
    CultureMech_006311 --> ing0_Columbia_agar_base
    ing1_Defibrinated_sheep_blood["Defibrinated sheep blood"]
    CultureMech_006311 --> ing1_Defibrinated_sheep_blood
    ing2_Casein_peptone["Casein peptone"]
    CultureMech_006311 --> ing2_Casein_peptone
    ing3_Soy_peptone["Soy peptone"]
    CultureMech_006311 --> ing3_Soy_peptone
    ing4_D____Glucose["D(+)-Glucose"]
    CultureMech_006311 --> ing4_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing4_D____Glucose -.-> CHEBI_17634
    ing5_NaCl["NaCl"]
    CultureMech_006311 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_K2HPO4["K2HPO4"]
    CultureMech_006311 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Calf_brains["Calf brains"]
    CultureMech_006311 --> ing7_Calf_brains
    ing8_Beef_heart["Beef heart"]
    CultureMech_006311 --> ing8_Beef_heart
    ing9_Proteose_peptone["Proteose peptone"]
    CultureMech_006311 --> ing9_Proteose_peptone
    ing10_Dextrose["Dextrose"]
    CultureMech_006311 --> ing10_Dextrose
    ing10_Dextrose -.-> CHEBI_17634
    ing11_Sodium_chloride["Sodium chloride"]
    CultureMech_006311 --> ing11_Sodium_chloride
    ing11_Sodium_chloride -.-> CHEBI_26710
    ing12_Disodium_phosphate["Disodium phosphate"]
    CultureMech_006311 --> ing12_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing12_Disodium_phosphate -.-> CHEBI_34683
    ing13_Peptone["Peptone"]
    CultureMech_006311 --> ing13_Peptone
    ing14_Meat_extract["Meat extract"]
    CultureMech_006311 --> ing14_Meat_extract

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Columbia agar base 1000 G_PER_L
2 Defibrinated sheep blood 50 G_PER_L
3 Casein peptone 17 G_PER_L
4 Soy peptone 3 G_PER_L
5 D(+)-Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17634
D-glucose
6 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
7 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Calf brains
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000955
brain
 infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
9 Beef heart
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000948
heart
 infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
10 Proteose peptone
Difco/BD Brain Heart Infusion 237500
10.0 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
 Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
11 Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
2.0 G_PER_L CHEBI:17634
D-glucose
 Carbon/energy source; Constituent of Brain Heart Infusion commercial product
12 Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
13 Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
2.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
14 Peptone 5 G_PER_L
15 Meat extract 3 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 15 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 693
2026-02-03T11:19:15.133000 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 693
2026-03-13T06:19:39.687530Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006311
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:56.833475+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "COLUMBIA BLOOD medium" to "columbia_blood_medium" for consistent matching
2026-06-05T07:40:25.452844+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:45.558215+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:57.515791+00:00 recipe-merger Merged 15 duplicate recipes into canonical record Sources: KOMODO_693_COLUMBIA_BLOOD_medium, columbia_blood_medium, medium_693_modified_for_dsm_13661, medium_693_modified_for_dsm_13760, medium_693_modified_for_dsm_18826, medium_693_modified_for_dsm_19736, medium_693_modified_for_dsm_20600, medium_693_modified_for_dsm_21031, medium_693_modified_for_dsm_21825, medium_693_modified_for_dsm_21843, medium_693_modified_for_dsm_21845, medium_693_modified_for_dsm_22221, medium_693_modified_for_dsm_22886, medium_693_modified_for_dsm_22909, medium_693_modified_for_dsm_23385
2026-08-06T03:04:57.515935+00:00 merge_recipes.py MERGED_RECIPES merged 15 source recipe(s) on fingerprint 3677278a753a635c4e41b6ec5bc9810e59a9aee30f5b951e0ec63b53a2648c0b