Overview
- External ontology
- komodo.medium:700 (source link unavailable) — ALKALIPHILIC SPIROCHAETE medium
- Applications
-
- Microbial cultivation
- pH
- 9.7
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 700 | DSMZ Medium: 700 (mediadive.medium:700) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006329["alkaliphilic_spirochaete_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_006329 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_006329 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_006329 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_006329 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_NH4Cl["NH4Cl"]
CultureMech_006329 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KCl["KCl"]
CultureMech_006329 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006329 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Yeast_extract["Yeast extract"]
CultureMech_006329 --> ing7_Yeast_extract
ing8_Sucrose["Sucrose"]
CultureMech_006329 --> ing8_Sucrose
CHEBI_17992["CHEBI:17992"]
ing8_Sucrose -.-> CHEBI_17992
ing9_Biotin["Biotin"]
CultureMech_006329 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_006329 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_006329 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Thiamine_HCl["Thiamine HCl"]
CultureMech_006329 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Riboflavin["Riboflavin"]
CultureMech_006329 --> ing13_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing13_Riboflavin -.-> CHEBI_17015
ing14_Nicotinic_acid["Nicotinic acid"]
CultureMech_006329 --> ing14_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing14_Nicotinic_acid -.-> CHEBI_15940
ing15_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_006329 --> ing15_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_Calcium_D_____pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_006329 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006329 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_006329 --> ing18__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing18__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_006329 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006329 --> ing20_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing20_MgSO4_x_7_H2O -.-> CHEBI_31795
ing21_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006329 --> ing21_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4_x_H2O -.-> CHEBI_86364
ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006329 --> ing22_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
ing23_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_006329 --> ing23_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing23_CoSO4_x_7_H2O -.-> CHEBI_91244
ing24_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006329 --> ing24_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing24_CaCl2_x_2_H2O -.-> CHEBI_86158
ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006329 --> ing25_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing26_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006329 --> ing26_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4_x_5_H2O -.-> CHEBI_31440
ing27_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_006329 --> ing27_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing27_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing28_H3BO3["H3BO3"]
CultureMech_006329 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006329 --> ing29_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213
CultureMech_006329 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
15 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
11.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 10.0, 1.0] |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
1 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 | Yeast extract | 0.5 G_PER_L | ||
| 9 |
Sucrose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17992
sucrose
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 14 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 15 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 16 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 22 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 23 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 24 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 25 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 26 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 27 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 28 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 30 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 31 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 32 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 33 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_700_ALKALIPHILIC_SPIROCHAETE_medium (CultureMech:006329)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 700 |
| 2026-02-03T11:19:04.460859 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 700 |
| 2026-02-06T05:10:28.787009Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:03.650055Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s); Added pH buffer: NaOH |
| 2026-03-13T06:19:39.764362Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006329 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.919857+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIPHILIC SPIROCHAETE medium" to "alkaliphilic_spirochaete_medium" for consistent matching |
| 2026-05-16T09:03:04.046184+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:25.512655+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.575592+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:57.641811+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d866a4c0f4f85b1404d4b7f4ef49ac2776ffdaf332fa6f57478a91cbf255bd40 |