Overview
- External ontology
- komodo.medium:761 (source link unavailable) — HALOTHERMOTHRIX ORENII
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 761 | DSMZ Medium: 761 (mediadive.medium:761) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006416["halothermothrix_orenii"]
ing0_NH4Cl["NH4Cl"]
CultureMech_006416 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_006416 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_006416 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006416 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006416 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_006416 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na_acetate["Na-acetate"]
CultureMech_006416 --> ing6_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Na_acetate -.-> CHEBI_32954
ing7_Glucose["Glucose"]
CultureMech_006416 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Trypticase["Trypticase"]
CultureMech_006416 --> ing8_Trypticase
ing9_NaCl["NaCl"]
CultureMech_006416 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_Resazurin["Resazurin"]
CultureMech_006416 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_006416 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006416 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006416 --> ing13_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing13_MnSO4_x_H2O -.-> CHEBI_86364
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006416 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_006416 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006416 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006416 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_006416 --> ing18_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing19_H3BO3["H3BO3"]
CultureMech_006416 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006416 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006416 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_006416 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.30000000000000004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.2, 0.1] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 9 | Trypticase | 0.5 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
101.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 100.0, 1.0] |
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Halothermothrix orenii
- Term
- Id
- NCBITaxon:373903
- Label
- Halothermothrix orenii H 168
- Genome assembly id
- GCA_000020485.1
- SAMN00623047
- Strain
- H168; H 168; DSM 9562; OCM 544
- Evidence
- Reference
- https://www.ncbi.nlm.nih.gov/bioproject/16377
- Supports
- SUPPORT
- Explanation
- NCBI BioProject PRJNA16377 identifies Halothermothrix orenii H168, Taxonomy ID 373903, complete genome assembly GCA_000020485.1, and BioSample SAMN00623047.
- Growth metrics
- Temperature celsius
- 60.0
- Measurement conditions
- Strict anaerobic cultivation in Halothermothrix orenii medium; source medium contained 100 g/L NaCl; parent medium has approximately 101 g/L NaCl and pH 7.0.
- Evidence
- Reference
- PMID:7520742
- Supports
- SUPPORT
- Snippet
- Strain H168 grew optimally in NaCl concentrations ranging from 50 to 100 g per liter
- Explanation
- The abstract directly reports growth of strain H168 at 60 C and optimum growth across 50-100 g/L NaCl, supporting a high-salt anaerobic variant of the parent Halothermothrix orenii medium.
- Is max attainment
- False
Recipe variants
- Name
- strict_anaerobic_halothermothrix_orenii_h168_growth
- Description
- DSMZ/KOMODO Halothermothrix orenii medium condition representing the original strain H168 thermohalophilic anaerobic growth evidence.
- Modifications
- Maintain strict anaerobic cultivation conditions.
- Use high-salt growth conditions centered on 100 g/L NaCl, matching the parent medium's approximately 101 g/L NaCl formulation.
- Incubate at 60 C for optimal growth of strain H168.
- Purpose
- Capture strain-specific growth evidence for Halothermothrix orenii H168 under the existing parent medium instead of creating a duplicate organism-named medium record.
- Evidence
- Reference
- PMID:7520742
- Supports
- SUPPORT
- Snippet
- A long rod, present only in the 40- to 60-cm layer, was isolated at 60 degrees C
- Explanation
- The source directly reports isolation of strain H168 at 60 C in a medium containing 100 g/L NaCl, which matches the high-salt Halothermothrix parent medium closely enough for a variant-level record.
- Reference
- https://www.ncbi.nlm.nih.gov/bioproject/16377
- Supports
- SUPPORT
- Explanation
- NCBI BioProject PRJNA16377 links Halothermothrix orenii H168 to complete genome assembly GCA_000020485.1 and BioSample SAMN00623047.
Provenance
Merged from 2 source records.
- KOMODO_761_HALOTHERMOTHRIX_ORENII (CultureMech:006416)
- halothermothrix_orenii (CultureMech:001894)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 761 |
| 2026-02-03T11:19:15.940154 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 761 |
| 2026-03-13T03:09:34.368580Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:40.564284Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006416 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:57.900183+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOTHERMOTHRIX ORENII" to "halothermothrix_orenii" for consistent matching |
| 2026-05-05T00:00:00+00:00 | literature_review_manual | ADDED_GROWTH_EVIDENCE | Added strain-specific thermohalophilic anaerobic growth evidence for Halothermothrix orenii H168 from PMID:7520742 and genome identifiers from NCBI BioProject PRJNA16377. |
| 2026-06-05T07:40:26.141740+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.777490+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T00:58:02.637595+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/8/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-09-22T00:08:55.061438+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_761_HALOTHERMOTHRIX_ORENII, halothermothrix_orenii |
| 2026-09-22T00:08:55.061474+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 7f6e2067db8bdd22cdb7e7a83ff27d47cd25968b3b86e5ea28b27f79e79acbcd |