gracilibacillus_medium

CultureMech:006519 bacterial COMPLEX LIQUID
Original name: GRACILIBACILLUS medium

Overview

External ontology
komodo.medium:802 (source link unavailable) — GRACILIBACILLUS medium
Applications
  • Microbial cultivation
pH
7.8
Notes
pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 802 | DSMZ Medium: 802 (mediadive.medium:802) | Aerobic: No

Composition

flowchart LR
    CultureMech_006519["gracilibacillus_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_006519 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006519 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KCl["KCl"]
    CultureMech_006519 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NaBr["NaBr"]
    CultureMech_006519 --> ing3_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing3_NaBr -.-> CHEBI_63004
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_006519 --> ing4_Yeast_extract
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_006519 --> ing5_Trypticase_peptone
    ing6_Tris_HCl["Tris-HCl"]
    CultureMech_006519 --> ing6_Tris_HCl
    ing7_KH2PO4["KH2PO4"]
    CultureMech_006519 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_CaCl2["CaCl2"]
    CultureMech_006519 --> ing8_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing8_CaCl2 -.-> CHEBI_3312
    ing9_Glucose["Glucose"]
    CultureMech_006519 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_HCl["HCl"]
    CultureMech_006519 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_006519 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006519 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006519 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_ZnCl2["ZnCl2"]
    CultureMech_006519 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_H3BO3["H3BO3"]
    CultureMech_006519 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006519 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006519 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006519 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_006519 --> ing19_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing19_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_006519 --> ing20_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing21_NaOH["NaOH"]
    CultureMech_006519 --> ing21_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing21_NaOH -.-> CHEBI_32145

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
4 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
5 Yeast extract 2 G_PER_L
6 Trypticase peptone 2 G_PER_L
7 Tris-HCl 12 G_PER_L
8 KH2PO4
H2O4P.K 136.084 g/mol
23.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 CaCl2
Ca.2Cl 110.984 g/mol
5 G_PER_L CHEBI:3312
calcium dichloride
10 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
11 HCl
HCl 36.461 g/mol
3.2 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
21.9802 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 [Merged 2 duplicates: 1.9802, 20.0]
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.247525 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
20.0990099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.0990099, 20.0]
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.0693069 G_PER_L CHEBI:49976
zinc dichloride
16 H3BO3
H3BO3 61.833 g/mol
0.00594059 G_PER_L CHEBI:33118
boric acid
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.039604 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0693069 G_PER_L CHEBI:34887
nickel dichloride
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0019802 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
0.0594059 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
21 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00594059 G_PER_L CHEBI:63939
sodium tungstate dihydrate
22 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 802
2026-02-03T11:19:02.328949 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 802
2026-02-06T05:10:24.923057Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:57.699066Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s); Added pH buffer: NaOH
2026-03-13T06:19:41.506406Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006519
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:58.976384+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GRACILIBACILLUS medium" to "gracilibacillus_medium" for consistent matching
2026-05-16T09:03:04.669613+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:26.569659+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:45.936387+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:59.540537+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 838d0aa5a98c702711607bb0ad36db8c61c05be62fa30b6b0b1d7670ef2d306c