Overview
- External ontology
- komodo.medium:847 (source link unavailable) — MACROMONAS medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.4}
- Notes
- Source: KOMODO ModelSEED | ID: 847 | DSMZ Medium: 847 (mediadive.medium:847) | Aerobic: No
Composition
flowchart LR
CultureMech_006654["macromonas_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_006654 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006654 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006654 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Yeast_extract["Yeast extract"]
CultureMech_006654 --> ing3_Yeast_extract
ing4_Agar["Agar"]
CultureMech_006654 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_006654 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_Disodium_succinate["Disodium succinate"]
CultureMech_006654 --> ing6_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing6_Disodium_succinate -.-> CHEBI_63675
ing7_Vitamin_B12["Vitamin B12"]
CultureMech_006654 --> ing7_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing7_Vitamin_B12 -.-> CHEBI_176843
ing8_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006654 --> ing8_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing8_p_Aminobenzoic_acid -.-> CHEBI_30753
ing9_D_____biotin["D-(+)-biotin"]
CultureMech_006654 --> ing9_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing9_D_____biotin -.-> CHEBI_15956
ing10_Nicotinic_acid["Nicotinic acid"]
CultureMech_006654 --> ing10_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing10_Nicotinic_acid -.-> CHEBI_15940
ing11_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006654 --> ing11_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing11_Calcium_pantothenate -.-> CHEBI_31345
ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_006654 --> ing12_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006654 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_006654 --> ing14_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing14_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_006654 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
ing16_ZnCl2["ZnCl2"]
CultureMech_006654 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006654 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_006654 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006654 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006654 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006654 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006654 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 | Yeast extract | 0.2 G_PER_L | ||
| 5 | Agar | 1 G_PER_L |
CHEBI:2509
agar
|
|
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.5 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 8 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 9 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 10 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 12 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 13 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_847_MACROMONAS_medium (CultureMech:006654)
- macromonas_medium (CultureMech:002004)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 847 |
| 2026-02-03T11:18:54.121858 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 847 |
| 2026-03-13T06:19:42.745240Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006654 |
| 2026-03-17T06:29:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.416185+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MACROMONAS medium" to "macromonas_medium" for consistent matching |
| 2026-05-20T06:00:54.280118+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4} |
| 2026-06-05T07:40:27.034750+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.096659+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:00.720180+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_847_MACROMONAS_medium, macromonas_medium |
| 2026-08-06T03:05:00.720228+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 82c396bdca9be267f5b27e43c118eaff81217bb3e1bd8e61e453bfb1b0f2f4e1 |