Overview
- External ontology
- komodo.medium:858 (source link unavailable) — ANAEROBIC CHOLESTEROL MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 858 | DSMZ Medium: 858 (mediadive.medium:858) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006675["anaerobic_cholesterol_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006675 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_NH4Cl["NH4Cl"]
CultureMech_006675 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_CaCl2["CaCl2"]
CultureMech_006675 --> ing2_CaCl2
CHEBI_3312["CHEBI:3312"]
ing2_CaCl2 -.-> CHEBI_3312
ing3_NaNO3["NaNO3"]
CultureMech_006675 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_KH2PO4["KH2PO4"]
CultureMech_006675 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NaHCO3["NaHCO3"]
CultureMech_006675 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_006675 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Pantothenic_acid["Pantothenic acid"]
CultureMech_006675 --> ing7_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing7_Pantothenic_acid -.-> CHEBI_7916
ing8_Riboflavin["Riboflavin"]
CultureMech_006675 --> ing8_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing8_Riboflavin -.-> CHEBI_17015
ing9_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_006675 --> ing9_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing9_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing10_Biotin["Biotin"]
CultureMech_006675 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_006675 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Nicotinic_acid["Nicotinic acid"]
CultureMech_006675 --> ing12_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing12_Nicotinic_acid -.-> CHEBI_15940
ing13_Nicotine_amide["Nicotine amide"]
CultureMech_006675 --> ing13_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing13_Nicotine_amide -.-> CHEBI_17154
ing14_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_006675 --> ing14_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing14_alpha_lipoic_acid -.-> CHEBI_16494
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006675 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006675 --> ing16_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing16_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing17_HCl["HCl"]
CultureMech_006675 --> ing17_HCl
CHEBI_17883["CHEBI:17883"]
ing17_HCl -.-> CHEBI_17883
ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_006675 --> ing18_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
ing19_ZnCl2["ZnCl2"]
CultureMech_006675 --> ing19_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing19_ZnCl2 -.-> CHEBI_49976
ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006675 --> ing20_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
ing21_H3BO3["H3BO3"]
CultureMech_006675 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006675 --> ing22_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
ing23_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006675 --> ing23_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing23_CuCl2_x_2_H2O -.-> CHEBI_86318
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006675 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006675 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing26_NaOH["NaOH"]
CultureMech_006675 --> ing26_NaOH
CHEBI_32145["CHEBI:32145"]
ing26_NaOH -.-> CHEBI_32145
ing27_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_006675 --> ing27_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing27_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_006675 --> ing28_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
0.8 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
84 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 9 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 10 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 15 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 17 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 18 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 19 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 20 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 21 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 24 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 27 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 28 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 29 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- anaerobic_cholesterol_medium (normalized source unresolved)
- anaerobic_cholesterol_medium (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 858 |
| 2026-02-03T11:19:15.235630 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 858 |
| 2026-03-13T06:19:42.897868Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006675 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.589392+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROBIC CHOLESTEROL MEDIUM" to "anaerobic_cholesterol_medium" for consistent matching |
| 2026-05-20T06:01:08.516586+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2} |
| 2026-06-05T07:40:48.876872+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.164239+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:23.403892+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: anaerobic_cholesterol_medium, anaerobic_cholesterol_medium |
| 2026-08-06T03:05:23.403941+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 762fff04d982bd4b67c732bf0ad88674d296c389d83436ef4867985a84a837c7 |