Overview
- External ontology
- komodo.medium:86_19627 (source link unavailable) — MEDIUM 86 MODIFIED FOR DSM 19627
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- pH buffer: Na-sesquicarbonate | Source: KOMODO ModelSEED | ID: 86_19627 | DSMZ Medium: 86 (mediadive.medium:86) | Aerobic: No
Composition
flowchart LR
CultureMech_006680["medium_86_modified_for_dsm_19627"]
ing0_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_006680 --> ing0_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing0_Nitrilotriacetic_acid -.-> CHEBI_44557
ing1_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_006680 --> ing1_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing1_CaSO4_x_2_H2O -.-> CHEBI_32583
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006680 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_006680 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KNO3["KNO3"]
CultureMech_006680 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_NaNO3["NaNO3"]
CultureMech_006680 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_006680 --> ing6_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_006680 --> ing7_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
ing8_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006680 --> ing8_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing8_MnSO4_x_H2O -.-> CHEBI_86364
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006680 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_H3BO3["H3BO3"]
CultureMech_006680 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006680 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006680 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006680 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_Tryptone["Tryptone"]
CultureMech_006680 --> ing14_Tryptone
ing15_Yeast_extract["Yeast extract"]
CultureMech_006680 --> ing15_Yeast_extract
ing16_Na["Na"]
CultureMech_006680 --> ing16_Na
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 2 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.06 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.008 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.103 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.689 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.14 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 8 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.00047 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 9 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0022 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0005 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.0005 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
2.5e-05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.5e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
4.6e-05 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 | Tryptone | 1 G_PER_L | ||
| 16 | Yeast extract | 1 G_PER_L | ||
| 17 | Na | variable VARIABLE | Extracted from recipe notes (pH buffer) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_86_modified_for_dsm_19627 (CultureMech:006680)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 86_19627 |
| 2026-02-03T11:18:54.712477 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 86 |
| 2026-03-13T06:19:42.930898Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006680 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.627691+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 86 MODIFIED FOR DSM 19627" to "medium_86_modified_for_dsm_19627" for consistent matching |
| 2026-05-16T09:04:19.398446+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:28.138878+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.378203+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:05.818563+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.821885+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 075173dd1e1b374c177f166251b270080250a4ed6b3ce5e0c9b21ee01378bdee |