modified_castenholz_medium

CultureMech:006702 bacterial COMPLEX LIQUID
Original name: MODIFIED CASTENHOLZ medium

Overview

External ontology
komodo.medium:86a (source link unavailable) — MODIFIED CASTENHOLZ medium
Applications
  • Microbial cultivation
pH
7.8
Notes
pH buffer: NAOH | Source: KOMODO ModelSEED | ID: 86a | DSMZ Medium: 86a (mediadive.medium:86a) | Aerobic: No

Composition

flowchart LR
    CultureMech_006702["modified_castenholz_medium"]
    ing0_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_006702 --> ing0_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing0_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing1_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_006702 --> ing1_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing1_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006702 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_006702 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KNO3["KNO3"]
    CultureMech_006702 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_NaNO3["NaNO3"]
    CultureMech_006702 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_006702 --> ing6_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_006702 --> ing7_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing8_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_006702 --> ing8_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing8_MnSO4_x_H2O -.-> CHEBI_86364
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_006702 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_H3BO3["H3BO3"]
    CultureMech_006702 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_006702 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006702 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006702 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_Tryptone["Tryptone"]
    CultureMech_006702 --> ing14_Tryptone
    ing15_Yeast_extract["Yeast extract"]
    CultureMech_006702 --> ing15_Yeast_extract
    ing16_NaOH["NaOH"]
    CultureMech_006702 --> ing16_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing16_NaOH -.-> CHEBI_32145

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.1 G_PER_L CHEBI:44557
nitrilotriacetic acid
2 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.06 G_PER_L CHEBI:32583
calcium sulfate dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NaCl
Cl.Na 58.443 g/mol
0.008 G_PER_L CHEBI:26710
sodium chloride
5 KNO3
K.NO3 101.102 g/mol
0.103 G_PER_L CHEBI:63043
potassium nitrate
6 NaNO3
NO3.Na 84.994 g/mol
0.689 G_PER_L CHEBI:63005
sodium nitrate
7 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.14 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
8 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.00047 G_PER_L CHEBI:86254
iron trichloride hexahydrate
9 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.0022 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0005 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.0005 G_PER_L CHEBI:33118
boric acid
12 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
2.5e-05 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
2.5e-05 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
4.6e-05 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 Tryptone 1 G_PER_L
16 Yeast extract 1 G_PER_L
17 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 86a
2026-02-03T11:18:58.064315 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 86
2026-03-13T03:08:46.937358Z data-quality-cleanup-v1.0 Data quality fixes Added pH buffer: NaOH
2026-03-13T06:19:43.147169Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006702
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:00.869838+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED CASTENHOLZ medium" to "modified_castenholz_medium" for consistent matching
2026-05-16T09:03:05.029617+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:27.155044+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.362982+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:46.132641+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:00.923560+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ab2c941918a1b0895b88a89983306ceb161a469de16616d6ad2ade084f892ca3