Overview
- External ontology
- komodo.medium:995 (source link unavailable) — NPB medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 995 | DSMZ Medium: 995 (mediadive.medium:995) | Aerobic: No
Composition
flowchart LR
CultureMech_006979["npb_medium"]
ing0_Tryptone_peptone["Tryptone peptone"]
CultureMech_006979 --> ing0_Tryptone_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_006979 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006979 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_K2HPO4["K2HPO4"]
CultureMech_006979 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_006979 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_D_Glucose["D-Glucose"]
CultureMech_006979 --> ing5_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing5_D_Glucose -.-> CHEBI_17634
ing6_Agar["Agar"]
CultureMech_006979 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_995_NPB_medium (CultureMech:006979)
- npb_medium (CultureMech:002180)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 995 |
| 2026-02-03T11:19:03.961507 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 995 |
| 2026-03-13T06:19:45.390650Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006979 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.453658+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NPB medium" to "npb_medium" for consistent matching |
| 2026-06-05T07:40:28.486275+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.635494+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:03.410213+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_995_NPB_medium, npb_medium |
| 2026-08-06T03:05:03.410254+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint f31c92c78ae455a81e71d96118b8cc56e08145c206a5fff99015889085351e2b |