Overview
- External ontology
- komodo.medium:996 (source link unavailable) — PERSEPHONELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 996 | DSMZ Medium: 996 (mediadive.medium:996) | Aerobic: No
Composition
flowchart LR
CultureMech_006980["persephonella_medium"]
ing0_NaCl["NaCl"]
CultureMech_006980 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NaOH["NaOH"]
CultureMech_006980 --> ing1_NaOH
CHEBI_32145["CHEBI:32145"]
ing1_NaOH -.-> CHEBI_32145
ing2_KCl["KCl"]
CultureMech_006980 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006980 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006980 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_006980 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006980 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NH4Cl["NH4Cl"]
CultureMech_006980 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_006980 --> ing8_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing9_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
CultureMech_006980 --> ing9_Na_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing9_Na_EDTA_x_2_H2O -.-> CHEBI_64758
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006980 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006980 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006980 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnCl2["ZnCl2"]
CultureMech_006980 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
CultureMech_006980 --> ing14_AlCl3_x_6_H2O
CHEBI_30115["CHEBI:30115"]
ing14_AlCl3_x_6_H2O -.-> CHEBI_30115
ing15_Na2O4W_x_2_H2O["Na2O4W x 2 H2O"]
CultureMech_006980 --> ing15_Na2O4W_x_2_H2O
ing16_CuCl["CuCl"]
CultureMech_006980 --> ing16_CuCl
CHEBI_53472["CHEBI:53472"]
ing16_CuCl -.-> CHEBI_53472
ing17_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_006980 --> ing17_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing17_NiSO4_x_6_H2O -.-> CHEBI_53001
ing18_H2SeO3["H2SeO3"]
CultureMech_006980 --> ing18_H2SeO3
CHEBI_26642["CHEBI:26642"]
ing18_H2SeO3 -.-> CHEBI_26642
ing19_H3BO3["H3BO3"]
CultureMech_006980 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006980 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_H2SO4["H2SO4"]
CultureMech_006980 --> ing21_H2SO4
CHEBI_26836["CHEBI:26836"]
ing21_H2SO4 -.-> CHEBI_26836
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
29 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
NaOH
HO.Na 39.997 g/mol
|
2 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1.36 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.2 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.5 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.15 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
|
0.04 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
|
| 16 | Na2O4W x 2 H2O | 0.03 G_PER_L | ||
| 17 |
CuCl
ClCu 98.999 g/mol
|
0.02 G_PER_L |
CHEBI:53472
copper(I) chloride
|
|
| 18 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.02 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 19 |
H2SeO3
H2O3Se 128.973 g/mol
|
0.01 G_PER_L |
CHEBI:26642
selenous acid
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_996_PERSEPHONELLA_MEDIUM (CultureMech:006980)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 996 |
| 2026-02-03T11:19:09.025850 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 996 |
| 2026-03-13T03:09:15.809604Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: H2SO4 |
| 2026-03-13T06:19:45.398687Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006980 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.462858+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PERSEPHONELLA MEDIUM" to "persephonella_medium" for consistent matching |
| 2026-05-16T09:03:05.904482+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:28.496447+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.637897+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:03.422086+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0979ecf0df553756b85abc4bc87f27a6d23092996d8b2acfbe80961785204879 |