mops_medium

CultureMech:006993 bacterial DEFINED LIQUID
Original name: Mops medium

Overview

External ontology
MEDIADB:107 (source link unavailable) — Mops medium
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_006993["mops_medium"]
    ing0_L_Methionine["L-Methionine"]
    CultureMech_006993 --> ing0_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing0_L_Methionine -.-> CHEBI_16643
    ing1_L_Leucine["L-Leucine"]
    CultureMech_006993 --> ing1_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing1_L_Leucine -.-> CHEBI_15603
    ing2_L_Proline["L-Proline"]
    CultureMech_006993 --> ing2_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing2_L_Proline -.-> CHEBI_17203
    ing3_L_Valine["L-Valine"]
    CultureMech_006993 --> ing3_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing3_L_Valine -.-> CHEBI_16414
    ing4_L_Threonine["L-Threonine"]
    CultureMech_006993 --> ing4_L_Threonine
    CHEBI_16857["CHEBI:16857"]
    ing4_L_Threonine -.-> CHEBI_16857
    ing5_Thiamine["Thiamine"]
    CultureMech_006993 --> ing5_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing5_Thiamine -.-> CHEBI_18385
    ing6_L_Isoleucine["L-Isoleucine"]
    CultureMech_006993 --> ing6_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing6_L_Isoleucine -.-> CHEBI_17191
    ing7_Magnesium_chloride["Magnesium chloride"]
    CultureMech_006993 --> ing7_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing7_Magnesium_chloride -.-> CHEBI_86345
    ing8_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_006993 --> ing8_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing8_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing9_Potassium_sulfate["Potassium sulfate"]
    CultureMech_006993 --> ing9_Potassium_sulfate
    CHEBI_32036["CHEBI:32036"]
    ing9_Potassium_sulfate -.-> CHEBI_32036
    ing10_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_006993 --> ing10_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing10_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing11_Sodium_chloride["Sodium chloride"]
    CultureMech_006993 --> ing11_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing11_Sodium_chloride -.-> CHEBI_26710
    ing12_Ammonium_chloride["Ammonium chloride"]
    CultureMech_006993 --> ing12_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing12_Ammonium_chloride -.-> CHEBI_31206
    ing13_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_006993 --> ing13_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing13_Ferrous_sulfate -.-> CHEBI_75832
    ing14_MOPS["MOPS"]
    CultureMech_006993 --> ing14_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing14_MOPS -.-> CHEBI_39074
    ing15_Tricine["Tricine"]
    CultureMech_006993 --> ing15_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing15_Tricine -.-> CHEBI_46760

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 L-Methionine
C5H11NO2S 149.215 g/mol
0.201 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
2 L-Leucine
C6H13NO2 131.175 g/mol
0.23 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
3 L-Proline
C5H9NO2 115.132 g/mol
0.26 MILLIMOLAR CHEBI:17203
L-proline
 Cross-references: KEGG:pro-L
4 L-Valine
C5H11NO2 117.148 g/mol
0.26 MILLIMOLAR CHEBI:16414
L-valine
 Cross-references: KEGG:val-L
5 L-Threonine
C4H9NO3 119.12 g/mol
0.25 MILLIMOLAR CHEBI:16857
L-threonine
 Cross-references: KEGG:thr-L
6 Thiamine
C12H17N4OS 265.362 g/mol
0.1 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
7 L-Isoleucine
C6H13NO2 131.175 g/mol
0.23 MILLIMOLAR CHEBI:17191
L-isoleucine
 Cross-references: KEGG:ile-L
8 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.523 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
9 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0005 MILLIMOLAR CHEBI:3312
calcium dichloride
10 Potassium sulfate
2K.O4S 174.259 g/mol
0.276 MILLIMOLAR CHEBI:32036
potassium sulfate
11 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
1.32 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
12 Sodium chloride
Cl.Na 58.443 g/mol
50.0 MILLIMOLAR CHEBI:26710
sodium chloride
13 Ammonium chloride
Cl.H4N 53.492 g/mol
9.52 MILLIMOLAR CHEBI:31206
ammonium chloride
14 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.01 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
15 MOPS 40.0 MILLIMOLAR CHEBI:39074
MOPS
16 Tricine 4.0 MILLIMOLAR CHEBI:46760
tricine

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:43.562630Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 107, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:25.701281Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.491598Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006993
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.570026+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Mops medium" to "mops_medium" for consistent matching
2026-06-05T07:41:38.241734+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:50.192339+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 8c86ddc9d8256cb2efd29e5cc79e73455ae768edae29814785b438d35f4beee5