Overview
- External ontology
- MEDIADB:121 (source link unavailable) — 'M9 with iptg (oh
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007009["m9_with_iptg_oh"]
ing0_beta_D_Glucose["beta-D-Glucose"]
CultureMech_007009 --> ing0_beta_D_Glucose
ing1_Ampicillin["Ampicillin"]
CultureMech_007009 --> ing1_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing1_Ampicillin -.-> CHEBI_28971
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007009 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007009 --> ing3_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing3_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_007009 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007009 --> ing5_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007009 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Ammonium_chloride["Ammonium chloride"]
CultureMech_007009 --> ing7_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing7_Ammonium_chloride -.-> CHEBI_31206
ing8_IPTG["IPTG"]
CultureMech_007009 --> ing8_IPTG
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | beta-D-Glucose | 11.1012 MILLIMOLAR | ||
| 2 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
0.2862 MILLIMOLAR |
CHEBI:28971
ampicillin
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.1 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
42.2654 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
|
8.55578 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 6 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
22.0449 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
1.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
18.6947 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 9 | IPTG | 0.2 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m9_with_iptg_oh (CultureMech:007009)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.680864Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 121, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.567732Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007009 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.655932+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'M9 with iptg (oh" to "m9_with_iptg_oh" for consistent matching |
| 2026-06-05T07:41:12.296188+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:41.412412+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 603909b503df8a5eb7cfa649ed911af38a72b5efe5e045b5d21b80ddcda80974 |