Overview
- External ontology
- MEDIADB:129 (source link unavailable) — 'acetate minimal media (zhao
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007017["acetate_minimal_media_zhao"]
ing0_Acetate["Acetate"]
CultureMech_007017 --> ing0_Acetate
CHEBI_30089["CHEBI:30089"]
ing0_Acetate -.-> CHEBI_30089
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007017 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007017 --> ing2_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing3_Sodium_molybdate["Sodium molybdate"]
CultureMech_007017 --> ing3_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing3_Sodium_molybdate -.-> CHEBI_75215
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_007017 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Thiamine_HCl["Thiamine HCl"]
CultureMech_007017 --> ing5_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing5_Thiamine_HCl -.-> CHEBI_49105
ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007017 --> ing6_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007017 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Cobalt_chloride["Cobalt chloride"]
CultureMech_007017 --> ing8_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing8_Cobalt_chloride -.-> CHEBI_35696
ing9_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007017 --> ing9_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing9_Ammonium_sulfate -.-> CHEBI_62946
ing10_Manganese_chloride["Manganese chloride"]
CultureMech_007017 --> ing10_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing10_Manganese_chloride -.-> CHEBI_63041
ing11_Cupric_chloride["Cupric chloride"]
CultureMech_007017 --> ing11_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing11_Cupric_chloride -.-> CHEBI_49553
ing12__Iron_III["'Iron(III"]
CultureMech_007017 --> ing12__Iron_III
ing13_Zinc_Chloride["Zinc Chloride"]
CultureMech_007017 --> ing13_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing13_Zinc_Chloride -.-> CHEBI_49976
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Acetate
C2H3O2 59.044 g/mol
|
83.263 MILLIMOLAR |
CHEBI:30089
acetate
|
Cross-references: KEGG:ac |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.04966 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
48.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.00248 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
|
10.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 6 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
3.32e-06 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 7 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
22.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
1.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.00252 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 10 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
10.0 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 11 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.00448 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 12 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.00252 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 13 | 'Iron(III | 0.06165 MILLIMOLAR | ||
| 14 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.01247 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- acetate_minimal_media_zhao (CultureMech:007017)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.757186Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 129, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.613263Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007017 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.706732+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'acetate minimal media (zhao" to "acetate_minimal_media_zhao" for consistent matching |
| 2026-06-05T07:40:46.628750+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:21.407114+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b25a2e31faa7606b34e260ee8d50db293097dec3303fc8d2fad7a28e5132012e |