Overview
- External ontology
- MEDIADB:145 (source link unavailable) — Glucose minimal media; Shehata et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007035["glucose_minimal_media_shehata_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007035 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_L_Methionine["L-Methionine"]
CultureMech_007035 --> ing1_L_Methionine
CHEBI_16643["CHEBI:16643"]
ing1_L_Methionine -.-> CHEBI_16643
ing2_Thymine["Thymine"]
CultureMech_007035 --> ing2_Thymine
CHEBI_17821["CHEBI:17821"]
ing2_Thymine -.-> CHEBI_17821
ing3_Magnesium_chloride["Magnesium chloride"]
CultureMech_007035 --> ing3_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing3_Magnesium_chloride -.-> CHEBI_86345
ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007035 --> ing4_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007035 --> ing5_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing6_Sodium_sulfate["Sodium sulfate"]
CultureMech_007035 --> ing6_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing6_Sodium_sulfate -.-> CHEBI_32149
ing7_Potassium_chloride["Potassium chloride"]
CultureMech_007035 --> ing7_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing7_Potassium_chloride -.-> CHEBI_32588
ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007035 --> ing8_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing9_Ammonium_chloride["Ammonium chloride"]
CultureMech_007035 --> ing9_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing9_Ammonium_chloride -.-> CHEBI_31206
ing10_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007035 --> ing10_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing10_Ferrous_sulfate -.-> CHEBI_75832
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
11.1012 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
L-Methionine
C5H11NO2S 149.215 g/mol
|
0.26807 MILLIMOLAR |
CHEBI:16643
L-methionine
|
Cross-references: KEGG:met-L |
| 3 |
Thymine
C5H6N2O2 126.115 g/mol
|
0.031717 MILLIMOLAR |
CHEBI:17821
thymine
|
Cross-references: KEGG:thym |
| 4 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.983719 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.0901024 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 6 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
48.7944 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
1.40805 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 8 |
Potassium chloride
Cl.K 74.551 g/mol
|
13.4136 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 9 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
30.8628 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
14.0211 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 11 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0017982 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- glucose_minimal_media_shehata_et_al (CultureMech:007035)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.897344Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 145, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:21.902144Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.712205Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007035 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.815848+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Glucose minimal media; Shehata et al" to "glucose_minimal_media_shehata_et_al" for consistent matching |
| 2026-06-05T07:41:06.461678+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:36.268458+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1dba68b0eec95544ae9864d89877114a5ae48b60766aa5c7f1fcbdb049809de0 |