Overview
- External ontology
- MEDIADB:147 (source link unavailable) — M9 medium; Fischer et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007037["m9_medium_fischer_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007037 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007037 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007037 --> ing2_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_007037 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Thiamine_HCl["Thiamine HCl"]
CultureMech_007037 --> ing4_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing4_Thiamine_HCl -.-> CHEBI_49105
ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007037 --> ing5_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007037 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Manganese_sulfate["Manganese sulfate"]
CultureMech_007037 --> ing7_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing7_Manganese_sulfate -.-> CHEBI_86364
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_007037 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Cobalt_chloride["Cobalt chloride"]
CultureMech_007037 --> ing9_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing9_Cobalt_chloride -.-> CHEBI_35696
ing10_Zinc_sulfate["Zinc sulfate"]
CultureMech_007037 --> ing10_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing10_Zinc_sulfate -.-> CHEBI_35176
ing11_Cupric_chloride["Cupric chloride"]
CultureMech_007037 --> ing11_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing11_Cupric_chloride -.-> CHEBI_49553
ing12__Iron_III["'Iron(III"]
CultureMech_007037 --> ing12__Iron_III
ing13_Sodium_EDTA["Sodium EDTA"]
CultureMech_007037 --> ing13_Sodium_EDTA
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
16.6518 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.1 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
52.9727 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
|
8.55578 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 5 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0033132 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 6 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
22.0449 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.00709975 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
14.9558 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.00756525 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 11 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.00626022 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 12 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.00703894 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 13 | 'Iron(III | 0.616722 MILLIMOLAR | ||
| 14 | Sodium EDTA | 0.597736 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m9_medium_fischer_et_al (CultureMech:007037)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.915038Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 147, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.723131Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007037 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.827468+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M9 medium; Fischer et al" to "m9_medium_fischer_et_al" for consistent matching |
| 2026-06-05T07:41:11.989537+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:41.146071+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4ea888e13fbdb1b00fc100c3ef044c6c003f4c597075e69e4b2eeba2958e7ad1 |