Overview
- External ontology
- MEDIADB:151 (source link unavailable) — MM-Succinate without biotin; Dunn et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007042["mm_succinate_without_biotin_dunn_et_al"]
ing0_Succinate["Succinate"]
CultureMech_007042 --> ing0_Succinate
CHEBI_26806["CHEBI:26806"]
ing0_Succinate -.-> CHEBI_26806
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007042 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007042 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Thiamine_HCl["Thiamine HCl"]
CultureMech_007042 --> ing3_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing3_Thiamine_HCl -.-> CHEBI_49105
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007042 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
ing5__Iron_III["'Iron(III"]
CultureMech_007042 --> ing5__Iron_III
ing6_Sodium_Glutamate["Sodium Glutamate"]
CultureMech_007042 --> ing6_Sodium_Glutamate
CHEBI_64220["CHEBI:64220"]
ing6_Sodium_Glutamate -.-> CHEBI_64220
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Succinate | 30.0 MILLIMOLAR |
CHEBI:26806
succinate
|
Cross-references: KEGG:succ |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.360425 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
1.2629 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.000331323 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.40572 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 6 | 'Iron(III | 0.123304 MILLIMOLAR | ||
| 7 |
Sodium Glutamate
C5H8NO4.Na 169.112 g/mol
|
6.50468 MILLIMOLAR |
CHEBI:64220
monosodium glutamate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mm_succinate_without_biotin_dunn_et_al (CultureMech:007042)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.951668Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 151, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:42.803845Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.748873Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007042 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.855386+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM-Succinate without biotin; Dunn et al" to "mm_succinate_without_biotin_dunn_et_al" for consistent matching |
| 2026-06-05T07:41:35.311846+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.956774+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5c6c6a11fb3710e1739eb226a256055e7cb4cb7a9ebc6f720e0cdfb0dc9dd96a |