Overview
- External ontology
- MEDIADB:153 (source link unavailable) — Mj media
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007044["mj_media"]
ing0_Urea["Urea"]
CultureMech_007044 --> ing0_Urea
CHEBI_16199["CHEBI:16199"]
ing0_Urea -.-> CHEBI_16199
ing1_Biotin["Biotin"]
CultureMech_007044 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_Cellobiose["Cellobiose"]
CultureMech_007044 --> ing2_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing2_Cellobiose -.-> CHEBI_17057
ing3_4_Aminobenzoate["4-Aminobenzoate"]
CultureMech_007044 --> ing3_4_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing3_4_Aminobenzoate -.-> CHEBI_17836
ing4_Vitamin_B12["Vitamin B12"]
CultureMech_007044 --> ing4_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing4_Vitamin_B12 -.-> CHEBI_176843
ing5_Magnesium_chloride["Magnesium chloride"]
CultureMech_007044 --> ing5_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing5_Magnesium_chloride -.-> CHEBI_86345
ing6_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007044 --> ing6_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing6_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing7_Resazurin["Resazurin"]
CultureMech_007044 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007044 --> ing8_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing8_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing9_Pyridoxamine_HCl["Pyridoxamine HCl"]
CultureMech_007044 --> ing9_Pyridoxamine_HCl
CHEBI_131531["CHEBI:131531"]
ing9_Pyridoxamine_HCl -.-> CHEBI_131531
ing10_Cysteine_HCl["Cysteine-HCl"]
CultureMech_007044 --> ing10_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing10_Cysteine_HCl -.-> CHEBI_91247
ing11_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007044 --> ing11_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing11_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing12_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007044 --> ing12_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing12_Ferrous_sulfate -.-> CHEBI_75832
ing13_Sodium_citrate["Sodium citrate"]
CultureMech_007044 --> ing13_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing13_Sodium_citrate -.-> CHEBI_53258
ing14_MOPS["MOPS"]
CultureMech_007044 --> ing14_MOPS
CHEBI_39074["CHEBI:39074"]
ing14_MOPS -.-> CHEBI_39074
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Urea
CH4N2O 60.056 g/mol
|
34.965 MILLIMOLAR |
CHEBI:16199
urea
|
Cross-references: KEGG:urea |
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.000819 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
| 3 |
Cellobiose
C12H22O11 342.297 g/mol
|
14.6071 MILLIMOLAR |
CHEBI:17057
cellobiose
|
|
| 4 |
4-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.002917 MILLIMOLAR |
CHEBI:17836
4-aminobenzoate
|
Cross-references: KEGG:4abz |
| 5 | Vitamin B12 | 0.0001476 MILLIMOLAR |
CHEBI:176843
vitamin B12
|
|
| 6 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
4.9186 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
1.0203 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00873 MILLIMOLAR |
CHEBI:8806
Resazurin
|
|
| 9 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
16.6475 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
Pyridoxamine HCl
C8H12N2O2.HCl 204.657 g/mol
|
0.00977 MILLIMOLAR |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 11 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
6.34437 MILLIMOLAR |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 12 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
11.0224 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.00481 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 14 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
10.2006 MILLIMOLAR |
CHEBI:53258
sodium citrate
|
|
| 15 | MOPS | 47.7867 MILLIMOLAR |
CHEBI:39074
MOPS
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mj_media (CultureMech:007044)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.968826Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 153, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:22.370745Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.760903Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007044 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.868486+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mj media" to "mj_media" for consistent matching |
| 2026-06-05T07:41:34.940865+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.655751+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9b62d5ce680023005b26831f5983b04355ef96a15db5ce87104327145805501e |