modified_m63_medium_with_hydroxyectoine_and_2_5_m_nacl

CultureMech:007050 bacterial DEFINED LIQUID
Original name: Modified m63 medium with hydroxyectoine and 2.5 m nacl

Overview

External ontology
MEDIADB:15 (source link unavailable) — Modified m63 medium with hydroxyectoine and 2.5 m nacl
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Synonyms
  • {'name': 'modified_m63_medium_with_hydroxyectoine_and_0_6_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:12', 'original_category': 'bacterial'}
  • {'name': 'modified_m63_medium_with_hydroxyectoine_and_0_75_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:13', 'original_category': 'bacterial'}
  • {'name': 'modified_m63_medium_with_hydroxyectoine_and_1_5_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:14', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007050["modified_m63_medium_with_hydroxyectoine_and_2_5_m_nacl"]
    ing0_Potassium_hydroxide["Potassium hydroxide"]
    CultureMech_007050 --> ing0_Potassium_hydroxide
    CHEBI_32035["CHEBI:32035"]
    ing0_Potassium_hydroxide -.-> CHEBI_32035
    ing1_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007050 --> ing1_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing1_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing2_5_Hydroxyectoine["5-Hydroxyectoine"]
    CultureMech_007050 --> ing2_5_Hydroxyectoine
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007050 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007050 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007050 --> ing5_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing5_Ferrous_sulfate -.-> CHEBI_75832
    ing6_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007050 --> ing6_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing6_Ammonium_sulfate -.-> CHEBI_62946

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Potassium hydroxide
HO.K 56.105 g/mol
75.0 MILLIMOLAR CHEBI:32035
potassium hydroxide
2 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
100.0 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
3 5-Hydroxyectoine 20.0 MILLIMOLAR
4 Sodium chloride
Cl.Na 58.443 g/mol
600.0 MILLIMOLAR CHEBI:26710
sodium chloride
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.16 MILLIMOLAR CHEBI:32599
magnesium sulfate
6 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0018 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
7 Ammonium sulfate
2H4N.O4S 132.141 g/mol
15.0 MILLIMOLAR CHEBI:62946
ammonium sulfate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-27T07:21:42.629940Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 15, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:45.794006Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007050
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.903621+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified m63 medium with hydroxyectoine and 2.5 m nacl" to "modified_m63_medium_with_hydroxyectoine_and_2_5_m_nacl" for consistent matching
2026-06-05T07:41:36.984161+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:48.685174+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: modified_m63_medium_with_hydroxyectoine_and_0_6_m_nacl, modified_m63_medium_with_hydroxyectoine_and_0_75_m_nacl, modified_m63_medium_with_hydroxyectoine_and_1_5_m_nacl, modified_m63_medium_with_hydroxyectoine_and_2_5_m_nacl
2026-08-06T03:05:48.685227+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint 8b175b7fa973e4be2e96f1732b036ce831e633d09fafaeaf00aa3a293a9b6f73