Overview
- External ontology
- MEDIADB:163 (source link unavailable) — M63; jebbar
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007054["m63_jebbar"]
ing0_Glycerol["Glycerol"]
CultureMech_007054 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Potassium_hydroxide["Potassium hydroxide"]
CultureMech_007054 --> ing1_Potassium_hydroxide
CHEBI_32035["CHEBI:32035"]
ing1_Potassium_hydroxide -.-> CHEBI_32035
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007054 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007054 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007054 --> ing4_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing4_Ferrous_sulfate -.-> CHEBI_75832
ing5_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007054 --> ing5_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing5_Ammonium_sulfate -.-> CHEBI_62946
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
22.0 MILLIMOLAR |
CHEBI:17754
glycerol
|
Cross-references: KEGG:glyc |
| 2 |
Potassium hydroxide
HO.K 56.105 g/mol
|
75.0 MILLIMOLAR |
CHEBI:32035
potassium hydroxide
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
100.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.16 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0039 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 6 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
15.0 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m63_jebbar (CultureMech:007054)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.042156Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 163, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.813811Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007054 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.925265+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M63; jebbar" to "m63_jebbar" for consistent matching |
| 2026-06-05T07:41:11.883051+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.978441+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0ae2570ab0df7551a68f4b551efe81193cf8fc9da15c80080e08219f56ddda45 |