Overview
- External ontology
- MEDIADB:166 (source link unavailable) — MOPS medium; VanBogelen
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007057["mops_medium_vanbogelen"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007057 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Thiamine["Thiamine"]
CultureMech_007057 --> ing1_Thiamine
CHEBI_18385["CHEBI:18385"]
ing1_Thiamine -.-> CHEBI_18385
ing2_Magnesium_chloride["Magnesium chloride"]
CultureMech_007057 --> ing2_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing2_Magnesium_chloride -.-> CHEBI_86345
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007057 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Potassium_sulfate["Potassium sulfate"]
CultureMech_007057 --> ing4_Potassium_sulfate
CHEBI_32036["CHEBI:32036"]
ing4_Potassium_sulfate -.-> CHEBI_32036
ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007057 --> ing5_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_007057 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Ammonium_chloride["Ammonium chloride"]
CultureMech_007057 --> ing7_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing7_Ammonium_chloride -.-> CHEBI_31206
ing8_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007057 --> ing8_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing8_Ferrous_sulfate -.-> CHEBI_75832
ing9_MOPS["MOPS"]
CultureMech_007057 --> ing9_MOPS
CHEBI_39074["CHEBI:39074"]
ing9_MOPS -.-> CHEBI_39074
ing10_Tricine["Tricine"]
CultureMech_007057 --> ing10_Tricine
CHEBI_46760["CHEBI:46760"]
ing10_Tricine -.-> CHEBI_46760
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
22.0 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.01 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 3 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.523 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.0005 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Potassium sulfate
2K.O4S 174.259 g/mol
|
0.276 MILLIMOLAR |
CHEBI:32036
potassium sulfate
|
|
| 6 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
1.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
|
50.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 8 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
9.52 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.01 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 | MOPS | 40.0 MILLIMOLAR |
CHEBI:39074
MOPS
|
|
| 11 | Tricine | 4.0 MILLIMOLAR |
CHEBI:46760
tricine
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mops_medium_vanbogelen (CultureMech:007057)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.066783Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 166, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:16.081712Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.828241Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007057 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.941306+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MOPS medium; VanBogelen" to "mops_medium_vanbogelen" for consistent matching |
| 2026-06-05T07:41:38.250052+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:50.203031+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 15c6e97714cfbbebc1b33070e036b9c60523f4c2827fc8c0cd2ac2b1b0a40bba |