Overview
- External ontology
- MEDIADB:167 (source link unavailable) — MM; Causey
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007058["mm_causey"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007058 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Thiamine["Thiamine"]
CultureMech_007058 --> ing1_Thiamine
CHEBI_18385["CHEBI:18385"]
ing1_Thiamine -.-> CHEBI_18385
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007058 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Boric_acid["Boric acid"]
CultureMech_007058 --> ing3_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing3_Boric_acid -.-> CHEBI_33118
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007058 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007058 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007058 --> ing6_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007058 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Cobalt_chloride["Cobalt chloride"]
CultureMech_007058 --> ing8_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing8_Cobalt_chloride -.-> CHEBI_35696
ing9_Cupric_chloride["Cupric chloride"]
CultureMech_007058 --> ing9_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing9_Cupric_chloride -.-> CHEBI_49553
ing10__Iron_III["'Iron(III"]
CultureMech_007058 --> ing10__Iron_III
ing11_Zinc_Chloride["Zinc Chloride"]
CultureMech_007058 --> ing11_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing11_Zinc_Chloride -.-> CHEBI_49976
ing12_Ammonium_phosphate["Ammonium phosphate"]
CultureMech_007058 --> ing12_Ammonium_phosphate
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
111.0 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.0017 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.1 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Boric acid
H3BO3 61.833 g/mol
|
0.00081 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
29.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.00097 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
26.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.1 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.00084 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 10 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.00074 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 11 | 'Iron(III | 0.01 MILLIMOLAR | ||
| 12 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.0015 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
|
| 13 | Ammonium phosphate | 27.0 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mm_causey (CultureMech:007058)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.074889Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 167, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.834102Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007058 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.947801+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM; Causey" to "mm_causey" for consistent matching |
| 2026-06-05T07:41:35.255569+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.883333+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3af6705d2f2c0ed82e1d0a370414ebdfcb0a5edfd96612a010b10cde258137b4 |