Overview
- External ontology
- MEDIADB:171 (source link unavailable) — MM-biotin and thiamine; Villasenor et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007062["mm_biotin_and_thiamine_villasenor_et_al"]
ing0_Succinate["Succinate"]
CultureMech_007062 --> ing0_Succinate
CHEBI_26806["CHEBI:26806"]
ing0_Succinate -.-> CHEBI_26806
ing1_Biotin["Biotin"]
CultureMech_007062 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_Thiamine["Thiamine"]
CultureMech_007062 --> ing2_Thiamine
CHEBI_18385["CHEBI:18385"]
ing2_Thiamine -.-> CHEBI_18385
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007062 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007062 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007062 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Ammonium_chloride["Ammonium chloride"]
CultureMech_007062 --> ing6_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing6_Ammonium_chloride -.-> CHEBI_31206
ing7__Iron_III["'Iron(III"]
CultureMech_007062 --> ing7__Iron_III
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Succinate | 10.0 MILLIMOLAR |
CHEBI:26806
succinate
|
Cross-references: KEGG:succ |
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.00409316 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
| 3 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.033243 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
1.49 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
1.26 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.83 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 7 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
10.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 8 | 'Iron(III | 0.0184 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mm_biotin_and_thiamine_villasenor_et_al (CultureMech:007062)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.113062Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 171, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.853154Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007062 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.968880+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM-biotin and thiamine; Villasenor et al" to "mm_biotin_and_thiamine_villasenor_et_al" for consistent matching |
| 2026-06-05T07:41:35.244188+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.875351+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c6a90ddf204363f42858e816b4deacd3f519ae74fac639215fba9725f5a89976 |