Overview
- External ontology
- MEDIADB:176 (source link unavailable) — Leitch et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Synonyms
-
- {'name': 'dawes_and_mandelstam_medium_from_leitch_et_al', 'source': 'unknown', 'source_id': 'MEDIADB:177', 'original_category': 'bacterial'}
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007067["leitch_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007067 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Tryptophan["Tryptophan"]
CultureMech_007067 --> ing1_Tryptophan
CHEBI_27897["CHEBI:27897"]
ing1_Tryptophan -.-> CHEBI_27897
ing2_Magnesium_chloride["Magnesium chloride"]
CultureMech_007067 --> ing2_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing2_Magnesium_chloride -.-> CHEBI_86345
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007067 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Sodium_sulfate["Sodium sulfate"]
CultureMech_007067 --> ing4_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing4_Sodium_sulfate -.-> CHEBI_32149
ing5_Potassium_chloride["Potassium chloride"]
CultureMech_007067 --> ing5_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing5_Potassium_chloride -.-> CHEBI_32588
ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007067 --> ing6_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing7_Ammonium_chloride["Ammonium chloride"]
CultureMech_007067 --> ing7_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing7_Ammonium_chloride -.-> CHEBI_31206
ing8_Manganese_chloride["Manganese chloride"]
CultureMech_007067 --> ing8_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing8_Manganese_chloride -.-> CHEBI_63041
ing9_Sodium_citrate["Sodium citrate"]
CultureMech_007067 --> ing9_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing9_Sodium_citrate -.-> CHEBI_53258
ing10__Iron_III["'Iron(III"]
CultureMech_007067 --> ing10__Iron_III
ing11_Sodium_nitrate["Sodium nitrate"]
CultureMech_007067 --> ing11_Sodium_nitrate
CHEBI_63005["CHEBI:63005"]
ing11_Sodium_nitrate -.-> CHEBI_63005
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
11.101 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Tryptophan
C11H12N2O2 204.229 g/mol
|
0.0097928 MILLIMOLAR |
CHEBI:27897
tryptophan
|
|
| 3 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.120997 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
1.0 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
1.0 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Potassium chloride
Cl.K 74.551 g/mol
|
1.006 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 7 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
9.99368 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
9.3473 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.39917 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 10 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
2.3352 MILLIMOLAR |
CHEBI:53258
sodium citrate
|
|
| 11 | 'Iron(III | 0.0039955 MILLIMOLAR | ||
| 12 |
Sodium nitrate
NO3.Na 84.994 g/mol
|
1.0 MILLIMOLAR |
CHEBI:63005
sodium nitrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- dawes_and_mandelstam_medium_from_leitch_et_al (CultureMech:007068)
- leitch_et_al (CultureMech:007067)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.159465Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 176, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:23.011097Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.883316Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007067 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.000120+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Leitch et al" to "leitch_et_al" for consistent matching |
| 2026-06-05T07:41:11.060925+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:29.635762+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: dawes_and_mandelstam_medium_from_leitch_et_al, leitch_et_al |
| 2026-08-06T03:05:29.635803+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 9499711a7fe88566e5bf8168906675c6ce8e3ed5a1ed423571d8620b19cf3876 |