minimal_medium_w_amino_acids_tang_et_al_2009

CultureMech:007075 bacterial DEFINED LIQUID
Original name: 'Minimal Medium w/amino acids (Tang et al 2009

Overview

External ontology
MEDIADB:184 (source link unavailable) — 'Minimal Medium w/amino acids (Tang et al 2009
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007075["minimal_medium_w_amino_acids_tang_et_al_2009"]
    ing0_L_Glutamate["L-Glutamate"]
    CultureMech_007075 --> ing0_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing0_L_Glutamate -.-> CHEBI_16015
    ing1_Glycine["Glycine"]
    CultureMech_007075 --> ing1_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing1_Glycine -.-> CHEBI_15428
    ing2_L_Methionine["L-Methionine"]
    CultureMech_007075 --> ing2_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing2_L_Methionine -.-> CHEBI_16643
    ing3_DL_Serine["DL-Serine"]
    CultureMech_007075 --> ing3_DL_Serine
    ing4_Cysteine["Cysteine"]
    CultureMech_007075 --> ing4_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing4_Cysteine -.-> CHEBI_15356
    ing5_Histidine["Histidine"]
    CultureMech_007075 --> ing5_Histidine
    CHEBI_27570["CHEBI:27570"]
    ing5_Histidine -.-> CHEBI_27570
    ing6_Alanine["Alanine"]
    CultureMech_007075 --> ing6_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing6_Alanine -.-> CHEBI_16977
    ing7_DL_Tyrosine["DL-Tyrosine"]
    CultureMech_007075 --> ing7_DL_Tyrosine
    CHEBI_18186["CHEBI:18186"]
    ing7_DL_Tyrosine -.-> CHEBI_18186
    ing8_Phenylalanine["Phenylalanine"]
    CultureMech_007075 --> ing8_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing8_Phenylalanine -.-> CHEBI_17295
    ing9_Arginine["Arginine"]
    CultureMech_007075 --> ing9_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing9_Arginine -.-> CHEBI_29016
    ing10_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007075 --> ing10_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing10_Magnesium_chloride -.-> CHEBI_86345
    ing11_Boric_acid["Boric acid"]
    CultureMech_007075 --> ing11_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing11_Boric_acid -.-> CHEBI_33118
    ing12_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007075 --> ing12_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing12_Sodium_sulfate -.-> CHEBI_32149
    ing13_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007075 --> ing13_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing13_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing14_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007075 --> ing14_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing14_Sodium_molybdate -.-> CHEBI_75215
    ing15_Aspartate["Aspartate"]
    CultureMech_007075 --> ing15_Aspartate
    ing16_Isoleucine["Isoleucine"]
    CultureMech_007075 --> ing16_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing16_Isoleucine -.-> CHEBI_17191
    ing17_Proline["Proline"]
    CultureMech_007075 --> ing17_Proline
    CHEBI_26271["CHEBI:26271"]
    ing17_Proline -.-> CHEBI_26271
    ing18_Valine["Valine"]
    CultureMech_007075 --> ing18_Valine
    CHEBI_16414["CHEBI:16414"]
    ing18_Valine -.-> CHEBI_16414
    ing19_Leucine["Leucine"]
    CultureMech_007075 --> ing19_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing19_Leucine -.-> CHEBI_15603
    ing20_Lysine["Lysine"]
    CultureMech_007075 --> ing20_Lysine
    ing21_Potassium_chloride["Potassium chloride"]
    CultureMech_007075 --> ing21_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing21_Potassium_chloride -.-> CHEBI_32588
    ing22_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007075 --> ing22_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing22_Ammonium_chloride -.-> CHEBI_31206
    ing23_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007075 --> ing23_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing23_Cobalt_chloride -.-> CHEBI_35696
    ing24_Manganese_chloride["Manganese chloride"]
    CultureMech_007075 --> ing24_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing24_Manganese_chloride -.-> CHEBI_63041
    ing25_Cupric_chloride["Cupric chloride"]
    CultureMech_007075 --> ing25_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing25_Cupric_chloride -.-> CHEBI_49553
    ing26_Sodium_selenite["Sodium selenite"]
    CultureMech_007075 --> ing26_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing26_Sodium_selenite -.-> CHEBI_48843
    ing27_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007075 --> ing27_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing27_Zinc_Chloride -.-> CHEBI_49976
    ing28_Ferrous_chloride["Ferrous chloride"]
    CultureMech_007075 --> ing28_Ferrous_chloride
    CHEBI_30812["CHEBI:30812"]
    ing28_Ferrous_chloride -.-> CHEBI_30812
    ing29_Sodium_tungstate["Sodium tungstate"]
    CultureMech_007075 --> ing29_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing29_Sodium_tungstate -.-> CHEBI_63940
    CultureMech_007075 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 L-Glutamate
C5H9NO4 147.13 g/mol
0.025 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
2 Glycine
C2H5NO2 75.067 g/mol
0.025 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
3 L-Methionine
C5H11NO2S 149.215 g/mol
0.025 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
4 DL-Serine 0.025 MILLIMOLAR
5 Cysteine
C3H7NO2S 121.161 g/mol
0.025 MILLIMOLAR CHEBI:15356
cysteine
6 Histidine
C6H9N3O2 155.157 g/mol
0.025 MILLIMOLAR CHEBI:27570
histidine
7 Alanine
C3H7NO2 89.094 g/mol
0.025 MILLIMOLAR CHEBI:16977
L-alanine
8 DL-Tyrosine
C9H11NO3 181.191 g/mol
0.025 MILLIMOLAR CHEBI:18186
tyrosine
9 Phenylalanine
C9H11NO2 165.192 g/mol
0.025 MILLIMOLAR CHEBI:17295
L-phenylalanine
10 Arginine
C6H14N4O2 174.204 g/mol
0.025 MILLIMOLAR CHEBI:29016
arginine
11 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
1.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
12 Boric acid
H3BO3 61.833 g/mol
0.0032346 MILLIMOLAR CHEBI:33118
boric acid
13 Sodium sulfate
2Na.O4S 142.043 g/mol
1.5 MILLIMOLAR CHEBI:32149
sodium sulfate
14 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
4.4 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
15 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.0017986 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
16 Aspartate 0.025 MILLIMOLAR
17 Isoleucine
C6H13NO2 131.175 g/mol
0.025 MILLIMOLAR CHEBI:17191
L-isoleucine
18 Proline
C5H9NO2 115.132 g/mol
0.025 MILLIMOLAR CHEBI:26271
proline
19 Valine
C5H11NO2 117.148 g/mol
0.025 MILLIMOLAR CHEBI:16414
L-valine
20 Leucine
C6H13NO2 131.175 g/mol
0.025 MILLIMOLAR CHEBI:15603
L-leucine
21 Lysine 0.025 MILLIMOLAR
22 Potassium chloride
Cl.K 74.551 g/mol
1.34 MILLIMOLAR CHEBI:32588
potassium chloride
23 Ammonium chloride
Cl.H4N 53.492 g/mol
28.0 MILLIMOLAR CHEBI:31206
ammonium chloride
24 Cobalt chloride
2Cl.Co 129.839 g/mol
0.014858 MILLIMOLAR CHEBI:35696
cobalt dichloride
25 Manganese chloride
Cl2Mn 125.844 g/mol
0.0224145 MILLIMOLAR CHEBI:63041
manganese(II) chloride
26 Cupric chloride
Cl2Cu 134.452 g/mol
0.000117316 MILLIMOLAR CHEBI:49553
copper(II) chloride
27 Sodium selenite
2Na.O3Se 172.937 g/mol
0.000228102 MILLIMOLAR CHEBI:48843
disodium selenite
28 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.0146718 MILLIMOLAR CHEBI:49976
zinc dichloride
29 Ferrous chloride
Cl2Fe 126.751 g/mol
0.0502992 MILLIMOLAR CHEBI:30812
iron dichloride
30 Sodium tungstate
2Na.O4W 293.816 g/mol
0.000242527 MILLIMOLAR CHEBI:63940
sodium tungstate
31 Nickel Sulfate 0.0038044 MILLIMOLAR
32 PIPES 10.0 MILLIMOLAR CHEBI:39033
PIPES
33 Sodium L-lactate
C3H5O3.Na 112.06 g/mol
30.0 MILLIMOLAR CHEBI:232798
sodium L-lactate
34 Threonine 0.025 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T07:21:44.247454Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 184, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:23.396460Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.965517Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007075
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.087902+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Minimal Medium w/amino acids (Tang et al 2009" to "minimal_medium_w_amino_acids_tang_et_al_2009" for consistent matching
2026-06-05T07:41:34.744604+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:46.440068+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3151b36806e04f7582d097761764de51371de40aa87d850570050859715eb489