Overview
- External ontology
- MEDIADB:185 (source link unavailable) — 'Minimal Medium w/amino acids and salt stress (Tang et al 2009
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007076["minimal_medium_w_amino_acids_and_salt_stress_tang_et_al_2009"]
ing0_L_Glutamate["L-Glutamate"]
CultureMech_007076 --> ing0_L_Glutamate
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamate -.-> CHEBI_16015
ing1_Glycine["Glycine"]
CultureMech_007076 --> ing1_Glycine
CHEBI_15428["CHEBI:15428"]
ing1_Glycine -.-> CHEBI_15428
ing2_L_Methionine["L-Methionine"]
CultureMech_007076 --> ing2_L_Methionine
CHEBI_16643["CHEBI:16643"]
ing2_L_Methionine -.-> CHEBI_16643
ing3_DL_Serine["DL-Serine"]
CultureMech_007076 --> ing3_DL_Serine
ing4_Cysteine["Cysteine"]
CultureMech_007076 --> ing4_Cysteine
CHEBI_15356["CHEBI:15356"]
ing4_Cysteine -.-> CHEBI_15356
ing5_Histidine["Histidine"]
CultureMech_007076 --> ing5_Histidine
CHEBI_27570["CHEBI:27570"]
ing5_Histidine -.-> CHEBI_27570
ing6_Alanine["Alanine"]
CultureMech_007076 --> ing6_Alanine
CHEBI_16977["CHEBI:16977"]
ing6_Alanine -.-> CHEBI_16977
ing7_DL_Tyrosine["DL-Tyrosine"]
CultureMech_007076 --> ing7_DL_Tyrosine
CHEBI_18186["CHEBI:18186"]
ing7_DL_Tyrosine -.-> CHEBI_18186
ing8_Phenylalanine["Phenylalanine"]
CultureMech_007076 --> ing8_Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing8_Phenylalanine -.-> CHEBI_17295
ing9_Arginine["Arginine"]
CultureMech_007076 --> ing9_Arginine
CHEBI_29016["CHEBI:29016"]
ing9_Arginine -.-> CHEBI_29016
ing10_Magnesium_chloride["Magnesium chloride"]
CultureMech_007076 --> ing10_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing10_Magnesium_chloride -.-> CHEBI_86345
ing11_Boric_acid["Boric acid"]
CultureMech_007076 --> ing11_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing11_Boric_acid -.-> CHEBI_33118
ing12_Sodium_sulfate["Sodium sulfate"]
CultureMech_007076 --> ing12_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing12_Sodium_sulfate -.-> CHEBI_32149
ing13_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007076 --> ing13_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing13_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing14_Sodium_molybdate["Sodium molybdate"]
CultureMech_007076 --> ing14_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing14_Sodium_molybdate -.-> CHEBI_75215
ing15_Aspartate["Aspartate"]
CultureMech_007076 --> ing15_Aspartate
ing16_Isoleucine["Isoleucine"]
CultureMech_007076 --> ing16_Isoleucine
CHEBI_17191["CHEBI:17191"]
ing16_Isoleucine -.-> CHEBI_17191
ing17_Proline["Proline"]
CultureMech_007076 --> ing17_Proline
CHEBI_26271["CHEBI:26271"]
ing17_Proline -.-> CHEBI_26271
ing18_Valine["Valine"]
CultureMech_007076 --> ing18_Valine
CHEBI_16414["CHEBI:16414"]
ing18_Valine -.-> CHEBI_16414
ing19_Leucine["Leucine"]
CultureMech_007076 --> ing19_Leucine
CHEBI_15603["CHEBI:15603"]
ing19_Leucine -.-> CHEBI_15603
ing20_Lysine["Lysine"]
CultureMech_007076 --> ing20_Lysine
ing21_Sodium_chloride["Sodium chloride"]
CultureMech_007076 --> ing21_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing21_Sodium_chloride -.-> CHEBI_26710
ing22_Potassium_chloride["Potassium chloride"]
CultureMech_007076 --> ing22_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing22_Potassium_chloride -.-> CHEBI_32588
ing23_Ammonium_chloride["Ammonium chloride"]
CultureMech_007076 --> ing23_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing23_Ammonium_chloride -.-> CHEBI_31206
ing24_Cobalt_chloride["Cobalt chloride"]
CultureMech_007076 --> ing24_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing24_Cobalt_chloride -.-> CHEBI_35696
ing25_Manganese_chloride["Manganese chloride"]
CultureMech_007076 --> ing25_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing25_Manganese_chloride -.-> CHEBI_63041
ing26_Cupric_chloride["Cupric chloride"]
CultureMech_007076 --> ing26_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing26_Cupric_chloride -.-> CHEBI_49553
ing27_Sodium_selenite["Sodium selenite"]
CultureMech_007076 --> ing27_Sodium_selenite
CHEBI_48843["CHEBI:48843"]
ing27_Sodium_selenite -.-> CHEBI_48843
ing28_Zinc_Chloride["Zinc Chloride"]
CultureMech_007076 --> ing28_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing28_Zinc_Chloride -.-> CHEBI_49976
ing29_Ferrous_chloride["Ferrous chloride"]
CultureMech_007076 --> ing29_Ferrous_chloride
CHEBI_30812["CHEBI:30812"]
ing29_Ferrous_chloride -.-> CHEBI_30812
CultureMech_007076 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamate
C5H9NO4 147.13 g/mol
|
0.025 MILLIMOLAR |
CHEBI:16015
L-glutamic acid
|
Cross-references: KEGG:glu-L |
| 2 |
Glycine
C2H5NO2 75.067 g/mol
|
0.025 MILLIMOLAR |
CHEBI:15428
glycine
|
Cross-references: KEGG:gly |
| 3 |
L-Methionine
C5H11NO2S 149.215 g/mol
|
0.025 MILLIMOLAR |
CHEBI:16643
L-methionine
|
Cross-references: KEGG:met-L |
| 4 | DL-Serine | 0.025 MILLIMOLAR | ||
| 5 |
Cysteine
C3H7NO2S 121.161 g/mol
|
0.025 MILLIMOLAR |
CHEBI:15356
cysteine
|
|
| 6 |
Histidine
C6H9N3O2 155.157 g/mol
|
0.025 MILLIMOLAR |
CHEBI:27570
histidine
|
|
| 7 |
Alanine
C3H7NO2 89.094 g/mol
|
0.025 MILLIMOLAR |
CHEBI:16977
L-alanine
|
|
| 8 |
DL-Tyrosine
C9H11NO3 181.191 g/mol
|
0.025 MILLIMOLAR |
CHEBI:18186
tyrosine
|
|
| 9 |
Phenylalanine
C9H11NO2 165.192 g/mol
|
0.025 MILLIMOLAR |
CHEBI:17295
L-phenylalanine
|
|
| 10 |
Arginine
C6H14N4O2 174.204 g/mol
|
0.025 MILLIMOLAR |
CHEBI:29016
arginine
|
|
| 11 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
1.0 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 12 |
Boric acid
H3BO3 61.833 g/mol
|
0.0032346 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 13 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
1.5 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 14 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
4.4 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 15 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.0017986 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 16 | Aspartate | 0.025 MILLIMOLAR | ||
| 17 |
Isoleucine
C6H13NO2 131.175 g/mol
|
0.025 MILLIMOLAR |
CHEBI:17191
L-isoleucine
|
|
| 18 |
Proline
C5H9NO2 115.132 g/mol
|
0.025 MILLIMOLAR |
CHEBI:26271
proline
|
|
| 19 |
Valine
C5H11NO2 117.148 g/mol
|
0.025 MILLIMOLAR |
CHEBI:16414
L-valine
|
|
| 20 |
Leucine
C6H13NO2 131.175 g/mol
|
0.025 MILLIMOLAR |
CHEBI:15603
L-leucine
|
|
| 21 | Lysine | 0.025 MILLIMOLAR | ||
| 22 |
Sodium chloride
Cl.Na 58.443 g/mol
|
330.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 23 |
Potassium chloride
Cl.K 74.551 g/mol
|
1.34 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 24 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
28.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 25 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.014858 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 26 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.0224145 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 27 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.000117316 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 28 |
Sodium selenite
2Na.O3Se 172.937 g/mol
|
0.000228102 MILLIMOLAR |
CHEBI:48843
disodium selenite
|
|
| 29 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.0146718 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
|
| 30 |
Ferrous chloride
Cl2Fe 126.751 g/mol
|
0.0502992 MILLIMOLAR |
CHEBI:30812
iron dichloride
|
|
| 31 |
Sodium tungstate
2Na.O4W 293.816 g/mol
|
0.000242527 MILLIMOLAR |
CHEBI:63940
sodium tungstate
|
|
| 32 | Nickel Sulfate | 0.0038044 MILLIMOLAR | ||
| 33 | PIPES | 10.0 MILLIMOLAR |
CHEBI:39033
PIPES
|
|
| 34 |
Sodium L-lactate
C3H5O3.Na 112.06 g/mol
|
30.0 MILLIMOLAR |
CHEBI:232798
sodium L-lactate
|
|
| 35 | Threonine | 0.025 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- minimal_medium_w_amino_acids_and_salt_stress_tang_et_al_2009 (CultureMech:007076)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T07:21:44.259619Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 185, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:08.382688Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.977960Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007076 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.100875+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'Minimal Medium w/amino acids and salt stress (Tang et al 2009" to "minimal_medium_w_amino_acids_and_salt_stress_tang_et_al_2009" for consistent matching |
| 2026-06-05T07:41:34.728580+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.418128+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0e920bbb8323c628d535e5d3c1cff2cf1414c4a8d446af3a9d5737ab97e1525f |