Overview
- External ontology
- MEDIADB:187 (source link unavailable) — LSG Medium
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007078["lsg_medium"]
ing0_Succinate["Succinate"]
CultureMech_007078 --> ing0_Succinate
CHEBI_26806["CHEBI:26806"]
ing0_Succinate -.-> CHEBI_26806
ing1_beta_D_Glucose["beta-D-Glucose"]
CultureMech_007078 --> ing1_beta_D_Glucose
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007078 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Boric_acid["Boric acid"]
CultureMech_007078 --> ing3_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing3_Boric_acid -.-> CHEBI_33118
ing4_Sodium_sulfate["Sodium sulfate"]
CultureMech_007078 --> ing4_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing4_Sodium_sulfate -.-> CHEBI_32149
ing5_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007078 --> ing5_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing6_Nickel_chloride["Nickel chloride"]
CultureMech_007078 --> ing6_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing6_Nickel_chloride -.-> CHEBI_34887
ing7_Sodium_molybdate["Sodium molybdate"]
CultureMech_007078 --> ing7_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing7_Sodium_molybdate -.-> CHEBI_75215
ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007078 --> ing8_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing9_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007078 --> ing9_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing9_Magnesium_sulfate -.-> CHEBI_32599
ing10_Ammonium_chloride["Ammonium chloride"]
CultureMech_007078 --> ing10_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing10_Ammonium_chloride -.-> CHEBI_31206
ing11_Cobalt_chloride["Cobalt chloride"]
CultureMech_007078 --> ing11_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing11_Cobalt_chloride -.-> CHEBI_35696
ing12_Manganese_chloride["Manganese chloride"]
CultureMech_007078 --> ing12_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing12_Manganese_chloride -.-> CHEBI_63041
ing13_Cupric_chloride["Cupric chloride"]
CultureMech_007078 --> ing13_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing13_Cupric_chloride -.-> CHEBI_49553
ing14_Sodium_selenite["Sodium selenite"]
CultureMech_007078 --> ing14_Sodium_selenite
CHEBI_48843["CHEBI:48843"]
ing14_Sodium_selenite -.-> CHEBI_48843
ing15__Iron_III["'Iron(III"]
CultureMech_007078 --> ing15__Iron_III
ing16_Zinc_Chloride["Zinc Chloride"]
CultureMech_007078 --> ing16_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing16_Zinc_Chloride -.-> CHEBI_49976
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Succinate | 20.0 MILLIMOLAR |
CHEBI:26806
succinate
|
Cross-references: KEGG:succ |
| 2 | beta-D-Glucose | 27.753 MILLIMOLAR | ||
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.004 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Boric acid
H3BO3 61.833 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 5 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
5.0 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
12.5 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 7 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 8 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 9 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
12.5 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 11 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
50.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 12 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 13 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.002 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 14 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 15 |
Sodium selenite
2Na.O3Se 172.937 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:48843
disodium selenite
|
|
| 16 | 'Iron(III | 0.01 MILLIMOLAR | ||
| 17 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.002 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lsg_medium (CultureMech:007078)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.277960Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 187, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.992871Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007078 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.117180+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LSG Medium" to "lsg_medium" for consistent matching |
| 2026-06-05T07:41:11.449027+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.594614+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d63eca3d192d3240fbcd01bc597f7d2ceca9b7180f18f0a5660bb07b55e915cc |