Overview
- External ontology
- MEDIADB:189 (source link unavailable) — PA-5052
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007079["pa_5052"]
ing0_L_Glutamate["L-Glutamate"]
CultureMech_007079 --> ing0_L_Glutamate
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamate -.-> CHEBI_16015
ing1_Glycine["Glycine"]
CultureMech_007079 --> ing1_Glycine
CHEBI_15428["CHEBI:15428"]
ing1_Glycine -.-> CHEBI_15428
ing2_L_Alanine["L-Alanine"]
CultureMech_007079 --> ing2_L_Alanine
CHEBI_16977["CHEBI:16977"]
ing2_L_Alanine -.-> CHEBI_16977
ing3_L_Lysine["L-Lysine"]
CultureMech_007079 --> ing3_L_Lysine
CHEBI_18019["CHEBI:18019"]
ing3_L_Lysine -.-> CHEBI_18019
ing4_L_Aspartate["L-Aspartate"]
CultureMech_007079 --> ing4_L_Aspartate
ing5_L_Arginine["L-Arginine"]
CultureMech_007079 --> ing5_L_Arginine
CHEBI_16467["CHEBI:16467"]
ing5_L_Arginine -.-> CHEBI_16467
ing6_L_Glutamine["L-Glutamine"]
CultureMech_007079 --> ing6_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing6_L_Glutamine -.-> CHEBI_18050
ing7_L_Serine["L-Serine"]
CultureMech_007079 --> ing7_L_Serine
CHEBI_17115["CHEBI:17115"]
ing7_L_Serine -.-> CHEBI_17115
ing8_L_Methionine["L-Methionine"]
CultureMech_007079 --> ing8_L_Methionine
CHEBI_16643["CHEBI:16643"]
ing8_L_Methionine -.-> CHEBI_16643
ing9_L_Tryptophan["L-Tryptophan"]
CultureMech_007079 --> ing9_L_Tryptophan
CHEBI_16828["CHEBI:16828"]
ing9_L_Tryptophan -.-> CHEBI_16828
ing10_L_Phenylalanine["L-Phenylalanine"]
CultureMech_007079 --> ing10_L_Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing10_L_Phenylalanine -.-> CHEBI_17295
ing11_Glycerol["Glycerol"]
CultureMech_007079 --> ing11_Glycerol
CHEBI_17754["CHEBI:17754"]
ing11_Glycerol -.-> CHEBI_17754
ing12_L_Leucine["L-Leucine"]
CultureMech_007079 --> ing12_L_Leucine
CHEBI_15603["CHEBI:15603"]
ing12_L_Leucine -.-> CHEBI_15603
ing13_L_Histidine["L-Histidine"]
CultureMech_007079 --> ing13_L_Histidine
CHEBI_15971["CHEBI:15971"]
ing13_L_Histidine -.-> CHEBI_15971
ing14_L_Proline["L-Proline"]
CultureMech_007079 --> ing14_L_Proline
CHEBI_17203["CHEBI:17203"]
ing14_L_Proline -.-> CHEBI_17203
ing15_L_Asparagine["L-Asparagine"]
CultureMech_007079 --> ing15_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing15_L_Asparagine -.-> CHEBI_17196
ing16_L_Valine["L-Valine"]
CultureMech_007079 --> ing16_L_Valine
CHEBI_16414["CHEBI:16414"]
ing16_L_Valine -.-> CHEBI_16414
ing17_L_Threonine["L-Threonine"]
CultureMech_007079 --> ing17_L_Threonine
CHEBI_16857["CHEBI:16857"]
ing17_L_Threonine -.-> CHEBI_16857
ing18_beta_D_Glucose["beta-D-Glucose"]
CultureMech_007079 --> ing18_beta_D_Glucose
ing19_Lactose["Lactose"]
CultureMech_007079 --> ing19_Lactose
CHEBI_17716["CHEBI:17716"]
ing19_Lactose -.-> CHEBI_17716
ing20_L_Isoleucine["L-Isoleucine"]
CultureMech_007079 --> ing20_L_Isoleucine
CHEBI_17191["CHEBI:17191"]
ing20_L_Isoleucine -.-> CHEBI_17191
ing21_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007079 --> ing21_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing21_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing22_Boric_acid["Boric acid"]
CultureMech_007079 --> ing22_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing22_Boric_acid -.-> CHEBI_33118
ing23_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007079 --> ing23_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing23_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing24_Nickel_chloride["Nickel chloride"]
CultureMech_007079 --> ing24_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing24_Nickel_chloride -.-> CHEBI_34887
ing25_Sodium_molybdate["Sodium molybdate"]
CultureMech_007079 --> ing25_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing25_Sodium_molybdate -.-> CHEBI_75215
ing26_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007079 --> ing26_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing26_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing27_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007079 --> ing27_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing27_Magnesium_sulfate -.-> CHEBI_32599
ing28_Cobalt_chloride["Cobalt chloride"]
CultureMech_007079 --> ing28_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing28_Cobalt_chloride -.-> CHEBI_35696
ing29_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007079 --> ing29_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing29_Ammonium_sulfate -.-> CHEBI_62946
CultureMech_007079 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamate
C5H9NO4 147.13 g/mol
|
1.35934 MILLIMOLAR |
CHEBI:16015
L-glutamic acid
|
Cross-references: KEGG:glu-L |
| 2 |
Glycine
C2H5NO2 75.067 g/mol
|
2.6641 MILLIMOLAR |
CHEBI:15428
glycine
|
Cross-references: KEGG:gly |
| 3 |
L-Alanine
C3H7NO2 89.094 g/mol
|
2.24492 MILLIMOLAR |
CHEBI:16977
L-alanine
|
Cross-references: KEGG:ala-L |
| 4 |
L-Lysine
C6H14N2O2 146.19 g/mol
|
3.4202 MILLIMOLAR |
CHEBI:18019
L-lysine
|
Cross-references: KEGG:lys-L |
| 5 | L-Aspartate | 1.5026 MILLIMOLAR | Cross-references: KEGG:asp-L | |
| 6 |
L-Arginine
C6H14N4O2 174.204 g/mol
|
1.1481 MILLIMOLAR |
CHEBI:16467
L-arginine
|
Cross-references: KEGG:arg-L |
| 7 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
1.36855 MILLIMOLAR |
CHEBI:18050
L-glutamine
|
Cross-references: KEGG:gln-L |
| 8 |
L-Serine
C3H7NO3 105.093 g/mol
|
1.9031 MILLIMOLAR |
CHEBI:17115
L-serine
|
Cross-references: KEGG:ser-L |
| 9 |
L-Methionine
C5H11NO2S 149.215 g/mol
|
1.34039 MILLIMOLAR |
CHEBI:16643
L-methionine
|
Cross-references: KEGG:met-L |
| 10 |
L-Tryptophan
C11H12N2O2 204.229 g/mol
|
0.97928 MILLIMOLAR |
CHEBI:16828
L-tryptophan
|
Cross-references: KEGG:trp-L |
| 11 |
L-Phenylalanine
C9H11NO2 165.192 g/mol
|
1.21073 MILLIMOLAR |
CHEBI:17295
L-phenylalanine
|
Cross-references: KEGG:phe-L |
| 12 |
Glycerol
C3H8O3 92.094 g/mol
|
54.294 MILLIMOLAR |
CHEBI:17754
glycerol
|
Cross-references: KEGG:glyc |
| 13 |
L-Leucine
C6H13NO2 131.175 g/mol
|
1.5247 MILLIMOLAR |
CHEBI:15603
L-leucine
|
Cross-references: KEGG:leu-L |
| 14 |
L-Histidine
C6H9N3O2 155.157 g/mol
|
1.28907 MILLIMOLAR |
CHEBI:15971
L-histidine
|
Cross-references: KEGG:his-L |
| 15 |
L-Proline
C5H9NO2 115.132 g/mol
|
1.73716 MILLIMOLAR |
CHEBI:17203
L-proline
|
Cross-references: KEGG:pro-L |
| 16 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
1.51377 MILLIMOLAR |
CHEBI:17196
L-asparagine
|
Cross-references: KEGG:asn-L |
| 17 |
L-Valine
C5H11NO2 117.148 g/mol
|
1.7072 MILLIMOLAR |
CHEBI:16414
L-valine
|
Cross-references: KEGG:val-L |
| 18 |
L-Threonine
C4H9NO3 119.12 g/mol
|
1.67897 MILLIMOLAR |
CHEBI:16857
L-threonine
|
Cross-references: KEGG:thr-L |
| 19 | beta-D-Glucose | 2.775 MILLIMOLAR | ||
| 20 |
Lactose
C12H22O11 342.297 g/mol
|
5.8428 MILLIMOLAR |
CHEBI:17716
lactose
|
Cross-references: KEGG:lcts |
| 21 |
L-Isoleucine
C6H13NO2 131.175 g/mol
|
1.5247 MILLIMOLAR |
CHEBI:17191
L-isoleucine
|
Cross-references: KEGG:ile-L |
| 22 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.004 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 23 |
Boric acid
H3BO3 61.833 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 24 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
50.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 25 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 27 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
50.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 28 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 29 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 30 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
25.0 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 31 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.002 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 32 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 33 |
Sodium selenite
2Na.O3Se 172.937 g/mol
|
0.0004 MILLIMOLAR |
CHEBI:48843
disodium selenite
|
|
| 34 | 'Iron(III | 0.01 MILLIMOLAR | ||
| 35 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.002 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pa_5052 (CultureMech:007079)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.285717Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 189, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.004559Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007079 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.129039+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PA-5052" to "pa_5052" for consistent matching |
| 2026-06-05T07:41:40.772992+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:52.175605+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7ad5b88f034933ead0757579a7c3d3308c2dbffe60b2ad185b2c3453adf98b78 |