m9_0_24_pyruvate

CultureMech:007082 bacterial DEFINED LIQUID
Original name: M9 0.24% pyruvate

Overview

External ontology
MEDIADB:191 (source link unavailable) — M9 0.24% pyruvate
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007082["m9_0_24_pyruvate"]
    ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007082 --> ing0_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing1_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007082 --> ing1_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing1_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_007082 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007082 --> ing3_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing3_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007082 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007082 --> ing5_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing5_Ammonium_chloride -.-> CHEBI_31206
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007082 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.1 MILLIMOLAR CHEBI:3312
calcium dichloride
2 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
47.743 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
3 Sodium chloride
Cl.Na 58.443 g/mol
8.5557 MILLIMOLAR CHEBI:26710
sodium chloride
4 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.045 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
2.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
6 Ammonium chloride
Cl.H4N 53.492 g/mol
18.6947 MILLIMOLAR CHEBI:31206
ammonium chloride
7 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
21.612 MILLIMOLAR CHEBI:50144
sodium pyruvate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:44.304137Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 191, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.023683Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007082
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.149502+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M9 0.24% pyruvate" to "m9_0_24_pyruvate" for consistent matching
2026-06-05T07:41:11.917281+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:41.020123+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 8264d5554d8aaade0d523f0310fd53e2042da3ccd82e0b94de67c309b7a43559