Overview
- External ontology
- MEDIADB:195 (source link unavailable) — Lr 0.4% glycerol
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007086["lr_0_4_glycerol"]
ing0_Glycerol["Glycerol"]
CultureMech_007086 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Sodium_hydroxide["Sodium hydroxide"]
CultureMech_007086 --> ing1_Sodium_hydroxide
CHEBI_32145["CHEBI:32145"]
ing1_Sodium_hydroxide -.-> CHEBI_32145
ing2_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007086 --> ing2_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing2_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007086 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007086 --> ing4_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing4_Ferrous_sulfate -.-> CHEBI_75832
ing5_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007086 --> ing5_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing5_Ammonium_sulfate -.-> CHEBI_62946
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
43.436 MILLIMOLAR |
CHEBI:17754
glycerol
|
Cross-references: KEGG:glyc |
| 2 |
Sodium hydroxide
HO.Na 39.997 g/mol
|
2.5006 MILLIMOLAR |
CHEBI:32145
sodium hydroxide
|
|
| 3 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
17.6359 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.02168 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.000108 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 6 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
0.12676 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lr_0_4_glycerol (CultureMech:007086)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.335338Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 195, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.041198Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007086 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.168649+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lr 0.4% glycerol" to "lr_0_4_glycerol" for consistent matching |
| 2026-06-05T07:41:11.421246+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.562845+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6731d53d1f342a05e42f9ae6b9480df71be172d65d18efd383aea7b01337de1e |