Overview
- External ontology
- MEDIADB:198 (source link unavailable) — Modified m9 with toluene
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007089["modified_m9_with_toluene"]
ing0_Toluene["Toluene"]
CultureMech_007089 --> ing0_Toluene
CHEBI_17578["CHEBI:17578"]
ing0_Toluene -.-> CHEBI_17578
ing1_Magnesium_chloride["Magnesium chloride"]
CultureMech_007089 --> ing1_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing1_Magnesium_chloride -.-> CHEBI_86345
ing2_Boric_acid["Boric acid"]
CultureMech_007089 --> ing2_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing2_Boric_acid -.-> CHEBI_33118
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007089 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Nickel_chloride["Nickel chloride"]
CultureMech_007089 --> ing4_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing4_Nickel_chloride -.-> CHEBI_34887
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007089 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_007089 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007089 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_007089 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Cobalt_chloride["Cobalt chloride"]
CultureMech_007089 --> ing9_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing9_Cobalt_chloride -.-> CHEBI_35696
ing10_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
CultureMech_007089 --> ing10_Sodium_dihydrogen_phosphate
CHEBI_37585["CHEBI:37585"]
ing10_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
ing11_Cupric_chloride["Cupric chloride"]
CultureMech_007089 --> ing11_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing11_Cupric_chloride -.-> CHEBI_49553
ing12_Zinc_Chloride["Zinc Chloride"]
CultureMech_007089 --> ing12_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing12_Zinc_Chloride -.-> CHEBI_49976
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Toluene
C7H8 92.141 g/mol
|
6.0 MILLIMOLAR |
CHEBI:17578
toluene
|
Cross-references: KEGG:tol |
| 2 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.00038 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
Boric acid
H3BO3 61.833 g/mol
|
0.013 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
220.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.00021 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.00031 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
|
86.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
4.3 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
187.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.0039 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 11 |
Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
|
194.0 MILLIMOLAR |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 12 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.00015 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 13 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.00092 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_m9_with_toluene (CultureMech:007089)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.360255Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 198, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:17.150009Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:46.057511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007089 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.186017+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified m9 with toluene" to "modified_m9_with_toluene" for consistent matching |
| 2026-06-05T07:41:37.010768+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-09T01:42:25.058299+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-08-06T03:05:48.718367+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a44adf80310d57dba5ca9635b091e49bdae1542de2c0f63388fef8bdce4aac58 |