Overview
- External ontology
- MEDIADB:201 (source link unavailable) — E2 medium with citrate
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007094["e2_medium_with_citrate"]
ing0_Citrate["Citrate"]
CultureMech_007094 --> ing0_Citrate
CHEBI_16947["CHEBI:16947"]
ing0_Citrate -.-> CHEBI_16947
ing1_Magnesium_chloride["Magnesium chloride"]
CultureMech_007094 --> ing1_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing1_Magnesium_chloride -.-> CHEBI_86345
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007094 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007094 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Cobaltous_sulfate["Cobaltous sulfate"]
CultureMech_007094 --> ing4_Cobaltous_sulfate
CHEBI_53470["CHEBI:53470"]
ing4_Cobaltous_sulfate -.-> CHEBI_53470
ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007094 --> ing5_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007094 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007094 --> ing7_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing7_Ferrous_sulfate -.-> CHEBI_75832
ing8_Zinc_sulfate["Zinc sulfate"]
CultureMech_007094 --> ing8_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing8_Zinc_sulfate -.-> CHEBI_35176
ing9_Cupric_chloride["Cupric chloride"]
CultureMech_007094 --> ing9_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing9_Cupric_chloride -.-> CHEBI_49553
ing10_Sodium_ammonium_phosphate["Sodium ammonium phosphate"]
CultureMech_007094 --> ing10_Sodium_ammonium_phosphate
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Citrate
C6H5O7 189.099 g/mol
|
13.0 MILLIMOLAR |
CHEBI:16947
citrate(3-)
|
Cross-references: KEGG:cit |
| 2 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.01 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.01 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
43.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Cobaltous sulfate
Co.O4S 154.996 g/mol
|
0.01 MILLIMOLAR |
CHEBI:53470
cobalt(2+) sulfate
|
|
| 6 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
27.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
1.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.01 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 9 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.01 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 10 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.01 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 11 | Sodium ammonium phosphate | 0.47 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- e2_medium_with_citrate (CultureMech:007094)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.385869Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 201, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:41.897885Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:46.099080Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007094 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.230200+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "E2 medium with citrate" to "e2_medium_with_citrate" for consistent matching |
| 2026-06-05T07:40:59.202455+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.221975+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7a83125430d9935b583dd7a42303b77f43f5c7196f5aa960435dbab5ad3849e6 |